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Improving LC–MS sensitivity through increases in chromatographic performance: Comparisons of UPLC–ES/MS/MS to HPLC–ES/MS/MS

Churchwell, M.; Twaddle, N.; Meeker, L.; Doerge, D.


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    "description": "Recent technological advances have made available reverse phase chromatographic\nmedia with a 1.7 micron particle size along with a liquid handling system that can operate\nsuch columns at much higher pressures. This technology, termed ultra performance\nliquid chromatography (UPLC), offers significant theoretical advantages in resolution,\nspeed, and sensitivity for analytical determinations, particularly when coupled with mass\nspectrometers capable of high-speed acquisitions. This paper explores the differences in\nLC/MS performance by conducting a side-by-side comparison of UPLC for several\nmethods previously optimized for HPLC-based separation and quantification of multiple\nanalytes with maximum throughput. In general, UPLC produced significant\nimprovements in method sensitivity, speed, and resolution. Sensitivity increases with\nUPLC, which were found to be analyte-dependent, were as large as 10- fold and\nimprovements in method speed were as large as 5- fold under conditions of comparable\npeak separations. Improvements in chromatographic resolution with UPLC were\napparent from generally narrower peak widths and from a separation of diastereomers not\npossible using HPLC. Overall, the improvements in LC/MS method sensitivity, speed,\nand resolution provided by UPLC show that further advances can be made in analytical\nmethodology to add significant value to hypothesis-driven research.", 
    "license": {
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    "title": "Improving LC\u2013MS sensitivity through increases in chromatographic performance: Comparisons of UPLC\u2013ES/MS/MS to HPLC\u2013ES/MS/MS", 
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        "name": "Churchwell, M."
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