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Progesterone metabolism in the pineal, brain stem, thalamus and corpus callosum of the female rat

Hanukoglu, Israel; Karavolas, Harry J.; Goy, Robert W.


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{
  "description": "Specific brain regions, namely, thalamus, tectum, tegmentum, cerebellum, medulla and pineal, from five proestrous rats were incubated for 30 min with [3H]progesterone. After reverse isotopic dilution analysis, the following metabolites were identified in all incubations by purification to constant specific activity, derivative formation and/or gas liquid chromatography trapping: [3H]5alpha-pregnane-3, 20-dione (10-20% of the starting substrate except pineal -- 0.7%), [3H]3alpha-hydroxy-5alpha-pregnan-20-one (1.6-3.8% except for pineal -- 0.5%) and [3H]20alpha-hydroxy-4-pregnen-3-one (0.05-0.11%). Preliminary results from the corpus collosum incubation indicated the presence of the same metabolites. Although some apparent constant specific activities were obtained for 20alpha-hydroxy-5alpha-pregnan-3-one and 5beta-pregnane-3, 20-dione, the low levels of 3H associated with these steroids did not permit a definitive identification. The results indicate the presence of at least delta1-steroid 5alpha-reductase, 3alpha-hydroxysteroid dehydrogenase and 20alpha-hydroxysteroid dehydrogenase activities with progesterone as substrate in the brain regions examined.", 
  "license": "", 
  "creator": [
    {
      "@id": "https://orcid.org/0000-0003-3889-0320", 
      "@type": "Person", 
      "name": "Hanukoglu, Israel"
    }, 
    {
      "@type": "Person", 
      "name": "Karavolas, Harry J."
    }, 
    {
      "@type": "Person", 
      "name": "Goy, Robert W."
    }
  ], 
  "headline": "Progesterone metabolism in the pineal, brain stem, thalamus and corpus callosum of the female rat", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "1977-04-01", 
  "url": "https://zenodo.org/record/890908", 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.1016/0006-8993(77)90624-2", 
  "@id": "https://doi.org/10.1016/0006-8993(77)90624-2", 
  "@type": "ScholarlyArticle", 
  "name": "Progesterone metabolism in the pineal, brain stem, thalamus and corpus callosum of the female rat"
}
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