Journal article Open Access

Progesterone metabolism in the pineal, brain stem, thalamus and corpus callosum of the female rat

Hanukoglu, Israel; Karavolas, Harry J.; Goy, Robert W.


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  <identifier identifierType="URL">https://zenodo.org/record/890908</identifier>
  <creators>
    <creator>
      <creatorName>Hanukoglu, Israel</creatorName>
      <givenName>Israel</givenName>
      <familyName>Hanukoglu</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-3889-0320</nameIdentifier>
    </creator>
    <creator>
      <creatorName>Karavolas, Harry J.</creatorName>
      <givenName>Harry J.</givenName>
      <familyName>Karavolas</familyName>
    </creator>
    <creator>
      <creatorName>Goy, Robert W.</creatorName>
      <givenName>Robert W.</givenName>
      <familyName>Goy</familyName>
    </creator>
  </creators>
  <titles>
    <title>Progesterone metabolism in the pineal, brain stem, thalamus and corpus callosum of the female rat</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>1977</publicationYear>
  <dates>
    <date dateType="Issued">1977-04-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/890908</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/0006-8993(77)90624-2</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Specific brain regions, namely, thalamus, tectum, tegmentum, cerebellum, medulla and pineal, from five proestrous rats were incubated for 30 min with [3H]progesterone. After reverse isotopic dilution analysis, the following metabolites were identified in all incubations by purification to constant specific activity, derivative formation and/or gas liquid chromatography trapping: [3H]5alpha-pregnane-3, 20-dione (10-20% of the starting substrate except pineal -- 0.7%), [3H]3alpha-hydroxy-5alpha-pregnan-20-one (1.6-3.8% except for pineal -- 0.5%) and [3H]20alpha-hydroxy-4-pregnen-3-one (0.05-0.11%). Preliminary results from the corpus collosum incubation indicated the presence of the same metabolites. Although some apparent constant specific activities were obtained for 20alpha-hydroxy-5alpha-pregnan-3-one and 5beta-pregnane-3, 20-dione, the low levels of 3H associated with these steroids did not permit a definitive identification. The results indicate the presence of at least delta1-steroid 5alpha-reductase, 3alpha-hydroxysteroid dehydrogenase and 20alpha-hydroxysteroid dehydrogenase activities with progesterone as substrate in the brain regions examined.</description>
  </descriptions>
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