Journal article Open Access

Progesterone metabolism in the pineal, brain stem, thalamus and corpus callosum of the female rat

Hanukoglu, Israel; Karavolas, Harry J.; Goy, Robert W.


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{
  "DOI": "10.1016/0006-8993(77)90624-2", 
  "author": [
    {
      "family": "Hanukoglu, Israel"
    }, 
    {
      "family": "Karavolas, Harry J."
    }, 
    {
      "family": "Goy, Robert W."
    }
  ], 
  "issued": {
    "date-parts": [
      [
        1977, 
        4, 
        1
      ]
    ]
  }, 
  "abstract": "Specific brain regions, namely, thalamus, tectum, tegmentum, cerebellum, medulla and pineal, from five proestrous rats were incubated for 30 min with [3H]progesterone. After reverse isotopic dilution analysis, the following metabolites were identified in all incubations by purification to constant specific activity, derivative formation and/or gas liquid chromatography trapping: [3H]5alpha-pregnane-3, 20-dione (10-20% of the starting substrate except pineal -- 0.7%), [3H]3alpha-hydroxy-5alpha-pregnan-20-one (1.6-3.8% except for pineal -- 0.5%) and [3H]20alpha-hydroxy-4-pregnen-3-one (0.05-0.11%). Preliminary results from the corpus collosum incubation indicated the presence of the same metabolites. Although some apparent constant specific activities were obtained for 20alpha-hydroxy-5alpha-pregnan-3-one and 5beta-pregnane-3, 20-dione, the low levels of 3H associated with these steroids did not permit a definitive identification. The results indicate the presence of at least delta1-steroid 5alpha-reductase, 3alpha-hydroxysteroid dehydrogenase and 20alpha-hydroxysteroid dehydrogenase activities with progesterone as substrate in the brain regions examined.", 
  "title": "Progesterone metabolism in the pineal, brain stem, thalamus and corpus callosum of the female rat", 
  "type": "article-journal", 
  "id": "890908"
}
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