835480
doi
10.1002/mas.21544
oai:zenodo.org:835480
Tabb, David L.
Stellenbosch University
Impens, Francis
Ghent University
Staes, An
Ghent University
Timmerman, Evy
Ghent University
Martens, Lennart
Ghent University
Laukens, Kris
Ghent University
Quality control in mass spectrometry-based proteomics
Bittremieux, Wout
University of Antwerp
info:eu-repo/semantics/openAccess
Creative Commons Attribution Share Alike 4.0 International
https://creativecommons.org/licenses/by-sa/4.0/legalcode
mass spectrometry
proteomics
quality control
<p>Mass spectrometry is a highly complex analytical technique and mass spectrometry-based proteomics experiments can be subject to a large variability, which forms an obstacle to obtaining accurate and reproducible results. Therefore, a comprehensive and systematic approach to quality control is an essential requirement to inspire confidence in the generated results.<br>
A typical mass spectrometry experiment consists of multiple different phases including the sample preparation, liquid chromatography, mass spectrometry, and bioinformatics stages. We review potential sources of variability that can impact the results of a mass spectrometry experiment occurring in all of these steps, and we discuss how to monitor and remedy the negative influences on the experimental results. Furthermore, we describe how specialized quality control samples of varying sample complexity can be incorporated into the experimental workflow and how they can be used to rigorously assess detailed aspects of the instrument performance.</p>
<p>This is the peer reviewed version of the following article: "Bittremieux, W., Tabb, D. L., Impens, F., Staes, A., Timmerman, E., Martens, L. & Laukens, K. Quality control in mass spectrometry-based proteomics. Mass Spectrometry Reviews (2017)", which has been published in final form at https://doi.org/10.1002/mas.21544. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.</p>
Zenodo
2017-07-26
info:eu-repo/semantics/article
835479
1579537927.849377
1861255
md5:111be0ca8de4c3d09c5f3a2903be9eac
https://zenodo.org/records/835480/files/Bittremieux_2017_Quality control in mass spectrometry-based proteomics.pdf
public
Mass Spectrometry Reviews
2017-07-26