Journal article Open Access

Prevalence and Molecular Detection of Quinolone-Resistant E. coli in Rectal Swab of Apparently Healthy Cattle in Bangladesh

Md. Montasir Mamun; Jayedul Hassan; K. H. M. Nazmul Hussain Nazir; Md. Alimul Islam; Khalada Zesmin; Md. Bahanur Rahman; Md. Tanvir Rahman


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    <subfield code="u">Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.</subfield>
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    <subfield code="u">Veterinary Training Institute, Mymensingh, Bangladesh.</subfield>
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    <subfield code="u">Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.</subfield>
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    <subfield code="u">Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.</subfield>
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    <subfield code="u">Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.</subfield>
    <subfield code="a">Md. Montasir Mamun</subfield>
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    <subfield code="a">Prevalence and Molecular Detection of Quinolone-Resistant E. coli in Rectal Swab of Apparently Healthy Cattle in Bangladesh</subfield>
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    <subfield code="a">&lt;p&gt;Emergence of antibiotic resistance is a serious health problem both in human and animal all over the world. In this study, we investigated the prevalence of quinolone-resistant &lt;em&gt;E. coli &lt;/em&gt;isolated from apparently healthy cattle in Mymensingh district, Bangladesh. A total of 137 rectal swabs was screened among which 95 was found positive for &lt;em&gt;E. coli. &lt;/em&gt;Confirmation of isolation of&lt;em&gt; E. coli&lt;/em&gt; was done by PCR targeting 16S rRNA gene of &lt;em&gt;E. coli &lt;/em&gt;(prevalence 69.3%). Resistance against quinolone is primarily due to activities of &lt;em&gt;qnrS&lt;/em&gt; and &lt;em&gt;qnrA&lt;/em&gt; gene products. Among these &lt;em&gt;E. coli &lt;/em&gt;quinolone-resistant gene &lt;em&gt;qnrS &lt;/em&gt;was detected in 11 isolates. None of the isolates were found positive for &lt;em&gt;qnr A&lt;/em&gt;gene. The overall prevalence of &lt;em&gt;qnrS&lt;/em&gt; positive &lt;em&gt;E. coli&lt;/em&gt; was 8.0%. Many of these quinolone-resistant &lt;em&gt;E. coli &lt;/em&gt;was multidrug-resistant. Nucleotide sequence analysis of &lt;em&gt;qnrS&lt;/em&gt; gene showed homology with the &lt;em&gt;qnrS&lt;/em&gt; gene detected in China, Nigeria, Taiwan, Russia, Turkey and USA. All isolates that were resistant to multiple quinolones were found highly sensitive to imipenem, ertapenem and meropenem. The results of this study indicated that apparently healthy cattle harbor quinolone-resistant &lt;em&gt;E. coli &lt;/em&gt;which have both clinical and public health significance&lt;em&gt;. &lt;/em&gt;If strict regulation on the use of quinolones in food animals is not maintained, these quinolone-resistant &lt;em&gt;E. coli &lt;/em&gt;may be transmitted to humans and other animals and may cause serious health problems in future. To the best of our knowledge, this is the first report on the molecular detection of quinolone-resistant &lt;em&gt;E. coli &lt;/em&gt;in cattle in Bangladesh.&lt;/p&gt;</subfield>
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