Optimizing Production of Mentha longifolia Essential Oil Emulsion Loaded with Omega 3 Fatty Acids by Nano-Fiber/Gas Chromatography

The main aim of this study is providing of Mentha longifolia L essential oil emulsion loaded with omega 3 fatty acids and optimization by polyaniline (PANI) nano-fiber/gas chromatography (PANI-F/GC). The head space solid phase microextraction (HS-SPME) method by polyaniline nanofibers was used to extract released essential oils of emulsion. The effects of four factors, including omega 3 percent, surfactant-to-oil ratio (SOR%), surfactant type (TWEEN 20, 80 and caseinate sodium) and storage time on the encapsulation efficiency, and essential oil chromatographic characteristics including total peak number, total peak area and total peak height were studied. The Central Composite Design (CCD) was used to design experiments and study the effects of four factors on the responses and P-value of <0.05 was considered statistically significant. Desirability function (D) was used for determination of optimum condition. Results showed that all four factors affected GC profile, essential oil release and encapsulation efficiency. According to the results the emulsion has the best release power on the condition: Omega 3 (%); 25, Storage Time (day); 60, SOR (%); 193.33 and Surfactant type; T80 where the total peak number was 3, total peak area: 407, total peak height: 77 and encapsulation efficiency was 75%.