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Multimodal imaging of subventricular zone neural stem/progenitor cells in the cuprizone mouse model reveals increased neurogenic potential for the olfactory bulb pathway, but no contribution to remyelination of the corpus callosum

Guglielmetti, Caroline; Praet, Jelle; Rangarajan, Janaki Raman; Vreys, Ruth; De Vocht, Nathalie; Maes, Frederik; Verhoye, Marleen; Ponsaerts, Peter; Van der Linden, Annemie


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{
  "description": "<p>Multiple sclerosis is a devastating demyelinating disease of the central nervous system(CNS) in which endogenous&nbsp;remyelination, and thus recovery, often fails. Although the cuprizone mouse model allowed elucidation of many molecular&nbsp;factors governing remyelination, currently very little is known about the spatial origin of the oligodendrocyte&nbsp;progenitor cells that initiate remyelination in this model. Therefore, we here investigated in this model whether&nbsp;subventricular zone (SVZ) neural stem/progenitor cells (NSPCs) contribute to remyelination of the splenium following&nbsp;cuprizone-induced demyelination. Experimentally, from the day of in situ NSPC labeling, C57BL/6J mice were fed&nbsp;a 0.2% cuprizone diet during a 4-week period and then left to recover on a normal diet for 8 weeks. Two in situ labeling&nbsp;strategies were employed: (i) NSPCs were labeled by intraventricular injection of micron-sized iron oxide&nbsp;particles and then followed up longitudinally by means of magnetic resonance imaging (MRI), and (ii) SVZ NSPCs&nbsp;were transduced with a lentiviral vector encoding the eGFP and Luciferase reporter proteins for longitudinal monitoring&nbsp;by means of in vivo bioluminescence imaging (BLI). In contrast to preceding suggestions, no migration of SVZ&nbsp;NSPC towards the demyelinated splenium was observed using both MRI and BLI, and further validated by histological&nbsp;analysis, thereby demonstrating that SVZ NSPCs are unable to contribute directly to remyelination of the&nbsp;splenium in the cuprizone model. Interestingly, using longitudinal BLI analysis and confirmed by histological analysis,&nbsp;an increased migration of SVZ NSPC-derived neuroblasts towards the olfactory bulb was observed following&nbsp;cuprizone treatment, indicative for a potential link between CNS inflammation and increased neurogenesis.</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Bio-Imaging Lab, University of Antwerp, Antwerp, Belgium", 
      "@type": "Person", 
      "name": "Guglielmetti, Caroline"
    }, 
    {
      "affiliation": "Bio-Imaging Lab, University of Antwerp, Antwerp, Belgium and Experimental Cell Transplantation Group, Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium", 
      "@type": "Person", 
      "name": "Praet, Jelle"
    }, 
    {
      "affiliation": "Medical Image Computing, ESAT/PSI, Department of Electrical Engineering, & Medical Imaging Research Center, KU Leuven & UZ Leuven, Belgium and iMinds Future Health Department, KU Leuven, Belgium", 
      "@type": "Person", 
      "name": "Rangarajan, Janaki Raman"
    }, 
    {
      "affiliation": "Bio-Imaging Lab, University of Antwerp, Antwerp, Belgium", 
      "@type": "Person", 
      "name": "Vreys, Ruth"
    }, 
    {
      "affiliation": "Bio-Imaging Lab, University of Antwerp, Antwerp, Belgium and Experimental Cell Transplantation Group, Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium", 
      "@type": "Person", 
      "name": "De Vocht, Nathalie"
    }, 
    {
      "affiliation": "Medical Image Computing, ESAT/PSI, Department of Electrical Engineering, & Medical Imaging Research Center, KU Leuven & UZ Leuven, Belgium and iMinds Future Health Department, KU Leuven, Belgium", 
      "@type": "Person", 
      "name": "Maes, Frederik"
    }, 
    {
      "affiliation": "Bio-Imaging Lab, University of Antwerp, Antwerp, Belgium", 
      "@type": "Person", 
      "name": "Verhoye, Marleen"
    }, 
    {
      "affiliation": "Experimental Cell Transplantation Group, Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium", 
      "@type": "Person", 
      "name": "Ponsaerts, Peter"
    }, 
    {
      "affiliation": "Bio-Imaging Lab, University of Antwerp, Antwerp, Belgium", 
      "@type": "Person", 
      "name": "Van der Linden, Annemie"
    }
  ], 
  "headline": "Multimodal imaging of subventricular zone neural stem/progenitor cells in the cuprizone mouse model reveals increased neurogenic potential for the olfactory bulb pathway, but no contribution to remyelination of the corpus callosum", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2013-08-07", 
  "url": "https://zenodo.org/record/7684", 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.1016/j.neuroimage.2013.07.080", 
  "@id": "https://doi.org/10.1016/j.neuroimage.2013.07.080", 
  "@type": "ScholarlyArticle", 
  "name": "Multimodal imaging of subventricular zone neural stem/progenitor cells in the cuprizone mouse model reveals increased neurogenic potential for the olfactory bulb pathway, but no contribution to remyelination of the corpus callosum"
}
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