Journal article Open Access

Multimodal imaging of subventricular zone neural stem/progenitor cells in the cuprizone mouse model reveals increased neurogenic potential for the olfactory bulb pathway, but no contribution to remyelination of the corpus callosum

Guglielmetti, Caroline; Praet, Jelle; Rangarajan, Janaki Raman; Vreys, Ruth; De Vocht, Nathalie; Maes, Frederik; Verhoye, Marleen; Ponsaerts, Peter; Van der Linden, Annemie


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  <identifier identifierType="URL">https://zenodo.org/record/7684</identifier>
  <creators>
    <creator>
      <creatorName>Guglielmetti, Caroline</creatorName>
      <givenName>Caroline</givenName>
      <familyName>Guglielmetti</familyName>
      <affiliation>Bio-Imaging Lab, University of Antwerp, Antwerp, Belgium</affiliation>
    </creator>
    <creator>
      <creatorName>Praet, Jelle</creatorName>
      <givenName>Jelle</givenName>
      <familyName>Praet</familyName>
      <affiliation>Bio-Imaging Lab, University of Antwerp, Antwerp, Belgium and Experimental Cell Transplantation Group, Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium</affiliation>
    </creator>
    <creator>
      <creatorName>Rangarajan, Janaki Raman</creatorName>
      <givenName>Janaki Raman</givenName>
      <familyName>Rangarajan</familyName>
      <affiliation>Medical Image Computing, ESAT/PSI, Department of Electrical Engineering, &amp; Medical Imaging Research Center, KU Leuven &amp; UZ Leuven, Belgium and iMinds Future Health Department, KU Leuven, Belgium</affiliation>
    </creator>
    <creator>
      <creatorName>Vreys, Ruth</creatorName>
      <givenName>Ruth</givenName>
      <familyName>Vreys</familyName>
      <affiliation>Bio-Imaging Lab, University of Antwerp, Antwerp, Belgium</affiliation>
    </creator>
    <creator>
      <creatorName>De Vocht, Nathalie</creatorName>
      <givenName>Nathalie</givenName>
      <familyName>De Vocht</familyName>
      <affiliation>Bio-Imaging Lab, University of Antwerp, Antwerp, Belgium and Experimental Cell Transplantation Group, Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium</affiliation>
    </creator>
    <creator>
      <creatorName>Maes, Frederik</creatorName>
      <givenName>Frederik</givenName>
      <familyName>Maes</familyName>
      <affiliation>Medical Image Computing, ESAT/PSI, Department of Electrical Engineering, &amp; Medical Imaging Research Center, KU Leuven &amp; UZ Leuven, Belgium and iMinds Future Health Department, KU Leuven, Belgium</affiliation>
    </creator>
    <creator>
      <creatorName>Verhoye, Marleen</creatorName>
      <givenName>Marleen</givenName>
      <familyName>Verhoye</familyName>
      <affiliation>Bio-Imaging Lab, University of Antwerp, Antwerp, Belgium</affiliation>
    </creator>
    <creator>
      <creatorName>Ponsaerts, Peter</creatorName>
      <givenName>Peter</givenName>
      <familyName>Ponsaerts</familyName>
      <affiliation>Experimental Cell Transplantation Group, Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium</affiliation>
    </creator>
    <creator>
      <creatorName>Van der Linden, Annemie</creatorName>
      <givenName>Annemie</givenName>
      <familyName>Van der Linden</familyName>
      <affiliation>Bio-Imaging Lab, University of Antwerp, Antwerp, Belgium</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Multimodal imaging of subventricular zone neural stem/progenitor cells in the cuprizone mouse model reveals increased neurogenic potential for the olfactory bulb pathway, but no contribution to remyelination of the corpus callosum</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2013</publicationYear>
  <dates>
    <date dateType="Issued">2013-08-07</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/7684</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.neuroimage.2013.07.080</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/inmind</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/ecfunded</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/zenodo</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;Multiple sclerosis is a devastating demyelinating disease of the central nervous system(CNS) in which endogenous&amp;nbsp;remyelination, and thus recovery, often fails. Although the cuprizone mouse model allowed elucidation of many molecular&amp;nbsp;factors governing remyelination, currently very little is known about the spatial origin of the oligodendrocyte&amp;nbsp;progenitor cells that initiate remyelination in this model. Therefore, we here investigated in this model whether&amp;nbsp;subventricular zone (SVZ) neural stem/progenitor cells (NSPCs) contribute to remyelination of the splenium following&amp;nbsp;cuprizone-induced demyelination. Experimentally, from the day of in situ NSPC labeling, C57BL/6J mice were fed&amp;nbsp;a 0.2% cuprizone diet during a 4-week period and then left to recover on a normal diet for 8 weeks. Two in situ labeling&amp;nbsp;strategies were employed: (i) NSPCs were labeled by intraventricular injection of micron-sized iron oxide&amp;nbsp;particles and then followed up longitudinally by means of magnetic resonance imaging (MRI), and (ii) SVZ NSPCs&amp;nbsp;were transduced with a lentiviral vector encoding the eGFP and Luciferase reporter proteins for longitudinal monitoring&amp;nbsp;by means of in vivo bioluminescence imaging (BLI). In contrast to preceding suggestions, no migration of SVZ&amp;nbsp;NSPC towards the demyelinated splenium was observed using both MRI and BLI, and further validated by histological&amp;nbsp;analysis, thereby demonstrating that SVZ NSPCs are unable to contribute directly to remyelination of the&amp;nbsp;splenium in the cuprizone model. Interestingly, using longitudinal BLI analysis and confirmed by histological analysis,&amp;nbsp;an increased migration of SVZ NSPC-derived neuroblasts towards the olfactory bulb was observed following&amp;nbsp;cuprizone treatment, indicative for a potential link between CNS inflammation and increased neurogenesis.&lt;/p&gt;</description>
  </descriptions>
  <fundingReferences>
    <fundingReference>
      <funderName>European Commission</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/501100000780</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/EC/FP7/278850/">278850</awardNumber>
      <awardTitle>Imaging of Neuroinflammation in Neurodegenerative Diseases</awardTitle>
    </fundingReference>
  </fundingReferences>
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