Journal article Open Access

Multimodal imaging of subventricular zone neural stem/progenitor cells in the cuprizone mouse model reveals increased neurogenic potential for the olfactory bulb pathway, but no contribution to remyelination of the corpus callosum

Guglielmetti, Caroline; Praet, Jelle; Rangarajan, Janaki Raman; Vreys, Ruth; De Vocht, Nathalie; Maes, Frederik; Verhoye, Marleen; Ponsaerts, Peter; Van der Linden, Annemie


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{
  "DOI": "10.1016/j.neuroimage.2013.07.080", 
  "container_title": "NeuroImage", 
  "title": "Multimodal imaging of subventricular zone neural stem/progenitor cells in the cuprizone mouse model reveals increased neurogenic potential for the olfactory bulb pathway, but no contribution to remyelination of the corpus callosum", 
  "issued": {
    "date-parts": [
      [
        2013, 
        8, 
        7
      ]
    ]
  }, 
  "abstract": "<p>Multiple sclerosis is a devastating demyelinating disease of the central nervous system(CNS) in which endogenous&nbsp;remyelination, and thus recovery, often fails. Although the cuprizone mouse model allowed elucidation of many molecular&nbsp;factors governing remyelination, currently very little is known about the spatial origin of the oligodendrocyte&nbsp;progenitor cells that initiate remyelination in this model. Therefore, we here investigated in this model whether&nbsp;subventricular zone (SVZ) neural stem/progenitor cells (NSPCs) contribute to remyelination of the splenium following&nbsp;cuprizone-induced demyelination. Experimentally, from the day of in situ NSPC labeling, C57BL/6J mice were fed&nbsp;a 0.2% cuprizone diet during a 4-week period and then left to recover on a normal diet for 8 weeks. Two in situ labeling&nbsp;strategies were employed: (i) NSPCs were labeled by intraventricular injection of micron-sized iron oxide&nbsp;particles and then followed up longitudinally by means of magnetic resonance imaging (MRI), and (ii) SVZ NSPCs&nbsp;were transduced with a lentiviral vector encoding the eGFP and Luciferase reporter proteins for longitudinal monitoring&nbsp;by means of in vivo bioluminescence imaging (BLI). In contrast to preceding suggestions, no migration of SVZ&nbsp;NSPC towards the demyelinated splenium was observed using both MRI and BLI, and further validated by histological&nbsp;analysis, thereby demonstrating that SVZ NSPCs are unable to contribute directly to remyelination of the&nbsp;splenium in the cuprizone model. Interestingly, using longitudinal BLI analysis and confirmed by histological analysis,&nbsp;an increased migration of SVZ NSPC-derived neuroblasts towards the olfactory bulb was observed following&nbsp;cuprizone treatment, indicative for a potential link between CNS inflammation and increased neurogenesis.</p>", 
  "author": [
    {
      "given": "Caroline", 
      "family": "Guglielmetti"
    }, 
    {
      "given": "Jelle", 
      "family": "Praet"
    }, 
    {
      "given": "Janaki Raman", 
      "family": "Rangarajan"
    }, 
    {
      "given": "Ruth", 
      "family": "Vreys"
    }, 
    {
      "given": "Nathalie", 
      "family": "De Vocht"
    }, 
    {
      "given": "Frederik", 
      "family": "Maes"
    }, 
    {
      "given": "Marleen", 
      "family": "Verhoye"
    }, 
    {
      "given": "Peter", 
      "family": "Ponsaerts"
    }, 
    {
      "given": "Annemie", 
      "family": "Van der Linden"
    }
  ], 
  "page": "99-110", 
  "volume": "86", 
  "type": "article-journal", 
  "id": "7684"
}
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