Wavelength (nm) norm(200nM M18 wt) norm(200nM Munc18-1 wt +340nM Syxntaxin-1a wt) norm(200nM Munc18-1 wt+ 340nM Syntaxin-1a delta 161-182) E F G H I J 310.00 68403 81865 74980 311.00 71167 85818 79304 312.00 73564 91418 84544 313.00 77677 95294 88127 314.00 79998 98553 93135 315.00 82480 1.0309e+05 96537 316.00 83622 1.0620e+05 99064 317.00 86170 1.0896e+05 1.0260e+05 318.00 86450 1.1166e+05 1.0429e+05 319.00 87638 1.1483e+05 1.0736e+05 320.00 89790 1.1743e+05 1.0883e+05 321.00 89672 1.1895e+05 1.1238e+05 322.00 90449 1.2165e+05 1.1254e+05 323.00 91885 1.2306e+05 1.1362e+05 324.00 91984 1.2513e+05 1.1543e+05 325.00 92284 1.2651e+05 1.1741e+05 326.00 92235 1.2675e+05 1.1879e+05 327.00 92570 1.2806e+05 1.1992e+05 328.00 92809 1.2842e+05 1.2010e+05 329.00 93064 1.2881e+05 1.2060e+05 330.00 91991 1.2934e+05 1.2055e+05 331.00 90983 1.2867e+05 1.2116e+05 332.00 90748 1.2846e+05 1.1947e+05 333.00 89494 1.2719e+05 1.1850e+05 334.00 87743 1.2722e+05 1.1696e+05 335.00 86113 1.2569e+05 1.1657e+05 336.00 85152 1.2465e+05 1.1479e+05 337.00 83596 1.2220e+05 1.1276e+05 338.00 82488 1.2007e+05 1.1174e+05 339.00 80705 1.1853e+05 1.0924e+05 340.00 78835 1.1624e+05 1.0806e+05 341.00 77448 1.1391e+05 1.0574e+05 342.00 74418 1.1182e+05 1.0289e+05 343.00 72640 1.0913e+05 1.0172e+05 344.00 71156 1.0706e+05 99384 345.00 70108 1.0542e+05 96835 346.00 68358 1.0248e+05 94498 347.00 66478 99812 92803 348.00 64884 97435 89710 349.00 63235 95259 88469 350.00 61621 92856 86229 351.00 59601 89972 83052 352.00 57708 87860 81213 353.00 55671 85097 78576 354.00 54369 82301 76227 355.00 52318 79256 73180 356.00 50154 77094 71769 357.00 48408 74264 69045 358.00 47595 71972 67290 359.00 45883 68855 65640 360.00 43897 67511 62081 361.00 42348 64291 60131 362.00 40639 62367 57904 363.00 39381 60592 55770 364.00 37946 57690 53513 365.00 36834 55290 51872 366.00 35201 53355 49928 367.00 33612 52010 47443 368.00 32606 49528 46400 369.00 31503 48026 44713 370.00 30513 46236 42834 371.00 29184 43866 40880 372.00 28046 42071 38931 373.00 26617 40529 37385 374.00 25540 38681 35397 375.00 24454 37077 33934 376.00 23396 35334 32725 377.00 24800 34009 31275 378.00 21129 32570 29919 379.00 20516 30952 28184 380.00 19511 29612 27507 381.00 18730 27824 25896 382.00 17890 26672 24511 383.00 17042 25401 23741 384.00 16134 24578 23016 385.00 15777 23245 21713 386.00 15025 22151 20823 387.00 14263 20647 19729 388.00 14968 20266 18761 389.00 13010 19081 18024 390.00 12510 18014 16770 391.00 11739 17378 16171 392.00 11214 16557 15574 393.00 10688 15890 14918 394.00 13945 15148 14165 395.00 10028 14470 13404 396.00 9393.1 13808 12732 397.00 8866.5 13329 12246 398.00 8869.5 12420 11508 399.00 8075.5 11664 11009 400.00 7903.2 11216 10360 401.00 7381.7 10754 9992.8 402.00 7001.3 9922.5 9441.1 403.00 6638.9 9602.2 8987.5 404.00 6292.6 9081.5 8460.9 405.00 5899.3 8899.3 8064.5 406.00 5827.1 8300.5 7856.1 407.00 5505.9 7755.0 7422.7 408.00 5222.7 7531.7 6835.1 409.00 5135.6 7050.3 6635.9 410.00 4814.4 8208.5 6241.6 411.00 4625.2 6254.6 6096.4 412.00 4233.9 6036.3 5788.1 413.00 4277.9 5855.1 5426.8 414.00 3868.7 5437.8 5230.7 415.00 3555.5 5338.6 4957.4 416.00 3579.5 4501.2 4547.2 417.00 3504.4 4637.1 4457.1 418.00 3393.3 4456.0 4266.9 419.00 3102.2 4263.8 6398.6 420.00 2729.0 4003.7 3932.7 421.00 2760.0 3828.5 3598.5 422.00 5493.8 3712.5 3471.4 423.00 2585.9 3357.3 3461.3 424.00 2457.8 3338.3 3200.2 425.00 2216.7 3244.2 2945.1 426.00 2358.8 3037.1 2948.0 427.00 2062.6 2914.0 2687.9 428.00 2073.6 2667.9 2418.8 429.00 1674.5 2456.8 2455.8 430.00 1974.6 2468.8 2148.7 431.00 1637.5 2343.7 2109.7 432.00 1602.5 2283.7 2077.7 433.00 1515.4 1970.6 2145.7 434.00 1600.4 1916.6 1960.6 435.00 1380.4 1814.5 1837.5 436.00 1188.3 1712.5 1515.4 437.00 1252.3 1605.4 1640.5 438.00 930.25 1698.5 1247.4 439.00 1052.3 2106.6 1477.4 440.00 1169.3 1568.4 1462.4 441.00 899.22 1439.4 1288.3 442.00 1058.3 1470.4 1289.3 443.00 1043.2 1143.3 1190.3 444.00 1021.2 1268.3 1123.3 445.00 927.21 1228.3 1117.3 446.00 866.20 1190.3 1114.3 447.00 856.19 1153.3 1064.3 448.00 698.15 993.22 944.22 449.00 714.16 995.22 894.21 450.00 718.15 1011.2 819.18 0.0000