Evaluation of antioxidant and anti-inflammatory effect of Resveratrol and BioPerine combination

ABSTRACT

The objective of the present work is to evaluate and establish the potential anti-inflammatory and antioxidant action of the Resveratrol and BioPerineÒ combination capsule available from Healthy Hey. The product responded positively to all the tests for flavonoids and alkaloids, confirming the presence of polyphenol and alkaloids in the capsule. The combination product was subjected to in vitro determination of antioxidant potential in terms of hydrogen donating or radical scavenging ability using the stable free radical DPPH, reducing power using potassium ferrocyanide, hydroxy radical scavenging using Iron-EDTA and ammonium molybdate methods. The IC₅₀ value of the Resveratrol and BioPerineÒ combination against DPPH was found to be 57.01µg/mL. The Resveratrol and BioPerineÒ combination was able to produce a dose and time time-dependent action potassium ferrocyanide to potassium ferricyanide. The maximum reduction occurred at 20 min post-interaction thereafter decreasing in the 30^th min. The IC₅₀ value of the Resveratrol and BioPerineÒ combination against the hydroxyl radical was found to be 106.79µg/mL. The IC₅₀ value of the Resveratrol and BioPerineÒ combination was found to be 154.88µg/mL. Resveratrol and BioPerineÒ combination was able to reduce the inhibition after the third hour significantly (p<0.01) exhibiting 50.87% reduction in edema volume.

Keywords: Resveratrol, BioPerine, antioxidant, anti-inflammatory, DPPH, Rat paw edema, carrageenan