Journal article Open Access

Effects of caffeinated energy drinks on cerebellum of Male albino rats

Syeda Sara Bano; Faiza Shafqat; Saeed Shafi; Ashraf Hussain; Sarwat Bukhari


Background: Energy drinks are widely being used by different age groups especially by teenagers in Pakistan. Higher level of caffeine in energy drinks have harmful effects on histology of brain tissue. Objective: This study aims to determine the impact of oral administration of red bull on cerebellum of adult male albino rats.  Study Design: Randomized Control trial. Place and Duration: Animal house National institute of Health Islamabad for 8 weeks Methodology: Red bull in dosage of 3.75 ml/kg body weight was administered by oral gavage daily for 4 weeks to experimental group B followed by normal diet for next 4 weeks. Experimental group C was given 3.75 ml/kg body weight of red bull for consecutive 8 weeks. The cerebellum was excised in each animal and weighed; fixed in formalin, stained with Hematoxylin and Eosin. Sections were observed for histological cytoarchitecture. Results: Results were taken in forms of photomicrographs and analyzed. Observations showed marked detachment of pia mater along with congestion and marked hypertrophy of molecular layer, distortion of purkinje cells and shrinkage of granular layer in experimental group C. Changes were minimal to mild in experimental group B.  Conclusion: Therefore, it is concluded that consumption of caffeinated energy drinks should be restricted to avoid their harmful effects on cerebellum leading to sleep disturbances and motor incoordination.

 Keywords: Caffeine, Energy drink, Cerebellum


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