Journal article Open Access

PHARMACOLOGICAL SCREENING OF ASPARAGUS RACEMOSUS FOR ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY BY EX-VIVO METHOD

Dhananjay, Dr Himanshu B Sahoo*

Object: The object of the present paper is pharmacological screening of Asparagus Racemosus and evaluated for the antioxidant and anti-inflammatory potential by Ex-vivo method.

Materials and method: The crude extract of Asparagus Racemosus was extracted out by the Soxhlet process. The quantitative chemical analysis was performed for the quantification and quality of active constituent. The antioxidant and anti-inflammatory activity of ethanolic extract of plant was determined using ex-vivo model. Antioxidant activity is assessed by the Hydrogen peroxide scavenging and reducing power assay method and Anti-inflammatory activity determined by the Protein denaturation assay.

Result and Discussion: The extract of Asparagus Racemosus was used for phytochemical constituent estimation and qualitative analysis. The Plant extract shown the sign of the alkaloid, saponin and tannins content. The ethanolic extract was found to be as tannin (15.12%), saponin (10.25%) and alkaloid (8.26%). The maximum hydrogen peroxide scavenging activity shown by ethanolic extract of Asparagus Racemosus was found to be 67.94 % at 400 µg/ml. In protein denaturation assay result stated that diclofenac sodium possesses maximum percentage of inhibition. The ethanolic extract of the plant Asparagus Racemosus possess significant inhibition activity at concentration 100, 200 and 400 µg/ml.

Conclusion: The ethanolic extract of Asparagus Racemosus showed dose dependent hydrogen peroxide scavenging activity. Reducing power of ethanolic extract of Asparagus Racemosus significantly increased with increasing concentration. The in-vitro anti-inflammatory potential of ethanolic extract of Asparagus Racemosus had shown concentration dependent inhibition of protein denaturation. The plant Asparagus Racemosus has shown promising antioxidant and anti-inflammatory activity without any side effects and will be beneficial for society and industry with standardization approaches.

Keywords: Asparagus Racemosus, Soxhlet, antioxidant, anti-inflammatoryprotein denaturation

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