Dataset Open Access

Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)

Alex L Payne-Dwyer; Aisha H Syeda; Mark C Leake

MARC21 XML Export

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nmm##2200000uu#4500</leader>
  <datafield tag="999" ind1="C" ind2="5">
    <subfield code="x">"RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live Escherichia coli with single-molecule sensitivity", Alex L. Payne-Dwyer, Aisha H. Syeda, Jack W. Shepherd, Lewis Frame, Mark. C. Leake bioRxiv 2021.10.04.463068; doi: https://doi.org/10.1101/2021.10.04.463068</subfield>
  </datafield>
  <datafield tag="041" ind1=" " ind2=" ">
    <subfield code="a">eng</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">DNA repair</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">single-molecule imaging</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">bacteria</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Slimfield</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">fluorescence microscopy</subfield>
  </datafield>
  <controlfield tag="005">20220614135107.0</controlfield>
  <controlfield tag="001">6639101</controlfield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">University of York</subfield>
    <subfield code="0">(orcid)0000-0003-3201-4135</subfield>
    <subfield code="a">Aisha H Syeda</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">University of York</subfield>
    <subfield code="0">(orcid)0000-0002-1715-1249</subfield>
    <subfield code="a">Mark C Leake</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">15827</subfield>
    <subfield code="z">md5:3e06c5f081684ebc0b4bd2083d039b3a</subfield>
    <subfield code="u">https://zenodo.org/record/6639101/files/Open Data Proforma Slimfield E. coli repair proteins (RecA-mGFP and RecB-sfGFP).docx</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">7135725900</subfield>
    <subfield code="z">md5:3bb5d51c1656a1946df61276b7e7b805</subfield>
    <subfield code="u">https://zenodo.org/record/6639101/files/RecAB raw Slimfield data.zip</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2022-06-13</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="p">openaire_data</subfield>
    <subfield code="o">oai:zenodo.org:6639101</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">University of York</subfield>
    <subfield code="0">(orcid)0000-0001-8802-352X</subfield>
    <subfield code="a">Alex L Payne-Dwyer</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)</subfield>
  </datafield>
  <datafield tag="536" ind1=" " ind2=" ">
    <subfield code="c">BB/P000746/1</subfield>
    <subfield code="a">Pushing proteins off DNA - how do helicases unwind protein-coated DNA?</subfield>
  </datafield>
  <datafield tag="536" ind1=" " ind2=" ">
    <subfield code="c">BB/N006453/1</subfield>
    <subfield code="a">Replication repair in real life: analysing how broken DNA replication machines are rebuilt inside cells.</subfield>
  </datafield>
  <datafield tag="536" ind1=" " ind2=" ">
    <subfield code="c">EP/T002166/1</subfield>
    <subfield code="a">Biological physics of protein clustering in epigenetic memory and transcriptional control</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">https://creativecommons.org/licenses/by/4.0/legalcode</subfield>
    <subfield code="a">Creative Commons Attribution 4.0 International</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="a">cc-by</subfield>
    <subfield code="2">opendefinition.org</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">&lt;p&gt;Imaging modality / instrument: &lt;em&gt;Brightfield&lt;/em&gt; + &lt;em&gt;Slimfield&lt;/em&gt;&lt;/p&gt;

&lt;p&gt;Image format:&lt;em&gt; OME TIFF (16 bit) + MicroManager metadata files&lt;/em&gt;&lt;/p&gt;

&lt;p&gt;Microscope settings:&lt;/p&gt;

&lt;p&gt;&lt;em&gt;488 nm triggered excitation; split detection, cropped to GFP or RFP/GFP (left/right) channels; 3 ms/frame laser exposure; Photometrics Prime95b CMOS&lt;/em&gt;&lt;/p&gt;

&lt;p&gt;Samples and acquisitions:&lt;/p&gt;

&lt;p&gt;Fluorescent fusions in live E.coli cells.&amp;nbsp; MMC = mitomycin C&lt;/p&gt;

&lt;table&gt;
	&lt;tbody&gt;
		&lt;tr&gt;
			&lt;td&gt;
			&lt;p&gt;No. fields of view&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;MMC-&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;MMC+ (0.5 ug/ml 3h)&lt;/p&gt;
			&lt;/td&gt;
		&lt;/tr&gt;
		&lt;tr&gt;
			&lt;td&gt;
			&lt;p&gt;RecA-mGFP&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;7&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;15&lt;/p&gt;
			&lt;/td&gt;
		&lt;/tr&gt;
		&lt;tr&gt;
			&lt;td&gt;
			&lt;p&gt;RecB-sfGFP&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;17&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;21&lt;/p&gt;
			&lt;/td&gt;
		&lt;/tr&gt;
		&lt;tr&gt;
			&lt;td&gt;
			&lt;p&gt;MG1655 control&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;10&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;10&lt;/p&gt;
			&lt;/td&gt;
		&lt;/tr&gt;
	&lt;/tbody&gt;
&lt;/table&gt;

&lt;p&gt;Approx. size before/after compression: 21 GB / 7 GB&lt;/p&gt;</subfield>
  </datafield>
  <datafield tag="773" ind1=" " ind2=" ">
    <subfield code="n">doi</subfield>
    <subfield code="i">isVersionOf</subfield>
    <subfield code="a">10.5281/zenodo.6639100</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.5281/zenodo.6639101</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">dataset</subfield>
  </datafield>
</record>
64
9
views
downloads
All versions This version
Views 6464
Downloads 99
Data volume 14.3 GB14.3 GB
Unique views 5151
Unique downloads 99
More info on how stats are collected.
Indexed in
Publication date:
June 13, 2022
DOI:
10.5281/zenodo.6639101

Zenodo DOI Badge

DOI 

10.5281/zenodo.6639101

Markdown 

[![DOI](https://zenodo.org/badge/DOI/10.5281/zenodo.6639101.svg)](https://doi.org/10.5281/zenodo.6639101)

reStructedText 

.. image:: https://zenodo.org/badge/DOI/10.5281/zenodo.6639101.svg
   :target: https://doi.org/10.5281/zenodo.6639101

HTML 

<a href="https://doi.org/10.5281/zenodo.6639101"><img src="https://zenodo.org/badge/DOI/10.5281/zenodo.6639101.svg" alt="DOI"></a>

Image URL 

https://zenodo.org/badge/DOI/10.5281/zenodo.6639101.svg

Target URL 

https://doi.org/10.5281/zenodo.6639101

Keyword(s):
DNA repair single-molecule imaging bacteria Slimfield fluorescence microscopy
Grants:
UK Research and Innovation:
  • Pushing proteins off DNA - how do helicases unwind protein-coated DNA? (BB/P000746/1)
  • Replication repair in real life: analysing how broken DNA replication machines are rebuilt inside cells. (BB/N006453/1)
  • Biological physics of protein clustering in epigenetic memory and transcriptional control (EP/T002166/1)
License (for files):
Creative Commons Attribution 4.0 International

Versions

Version 1.0 10.5281/zenodo.6639101 Jun 13, 2022
Cite all versions? You can cite all versions by using the DOI 10.5281/zenodo.6639100. This DOI represents all versions, and will always resolve to the latest one. Read more.

Share

Cite as

Export