June 13, 2022 Dataset Open Access

Alex L Payne-Dwyer; Aisha H Syeda; Mark C Leake

Dublin Core Export

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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Alex L Payne-Dwyer</dc:creator>
  <dc:creator>Aisha H Syeda</dc:creator>
  <dc:creator>Mark C Leake</dc:creator>
  <dc:date>2022-06-13</dc:date>
  <dc:description>Imaging modality / instrument: Brightfield + Slimfield

Image format: OME TIFF (16 bit) + MicroManager metadata files

Microscope settings:

488 nm triggered excitation; split detection, cropped to GFP or RFP/GFP (left/right) channels; 3 ms/frame laser exposure; Photometrics Prime95b CMOS

Samples and acquisitions:

Fluorescent fusions in live E.coli cells.  MMC = mitomycin C


	
		
			
			No. fields of view
			
			
			MMC-
			
			
			MMC+ (0.5 ug/ml 3h)
			
		
		
			
			RecA-mGFP
			
			
			7
			
			
			15
			
		
		
			
			RecB-sfGFP
			
			
			17
			
			
			21
			
		
		
			
			MG1655 control
			
			
			10
			
			
			10
			
		
	


Approx. size before/after compression: 21 GB / 7 GB</dc:description>
  <dc:identifier>https://zenodo.org/record/6639101</dc:identifier>
  <dc:identifier>10.5281/zenodo.6639101</dc:identifier>
  <dc:identifier>oai:zenodo.org:6639101</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/grantAgreement/UKRI/BBSRC/BB/P000746/1/</dc:relation>
  <dc:relation>info:eu-repo/grantAgreement/UKRI/BBSRC/BB/N006453/1/</dc:relation>
  <dc:relation>info:eu-repo/grantAgreement/UKRI/EPSRC/EP/T002166/1/</dc:relation>
  <dc:relation>doi:10.5281/zenodo.6639100</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>https://creativecommons.org/licenses/by/4.0/legalcode</dc:rights>
  <dc:subject>DNA repair</dc:subject>
  <dc:subject>single-molecule imaging</dc:subject>
  <dc:subject>bacteria</dc:subject>
  <dc:subject>Slimfield</dc:subject>
  <dc:subject>fluorescence microscopy</dc:subject>
  <dc:title>Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)</dc:title>
  <dc:type>info:eu-repo/semantics/other</dc:type>
  <dc:type>dataset</dc:type>
</oai_dc:dc>