June 13, 2022 Dataset Open Access

Alex L Payne-Dwyer; Aisha H Syeda; Mark C Leake

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{
  "inLanguage": {
    "alternateName": "eng", 
    "@type": "Language", 
    "name": "English"
  }, 
  "description": "<p>Imaging modality / instrument: <em>Brightfield</em> + <em>Slimfield</em></p>\n\n<p>Image format:<em> OME TIFF (16 bit) + MicroManager metadata files</em></p>\n\n<p>Microscope settings:</p>\n\n<p><em>488 nm triggered excitation; split detection, cropped to GFP or RFP/GFP (left/right) channels; 3 ms/frame laser exposure; Photometrics Prime95b CMOS</em></p>\n\n<p>Samples and acquisitions:</p>\n\n<p>Fluorescent fusions in live E.coli cells.&nbsp; MMC = mitomycin C</p>\n\n<table>\n\t<tbody>\n\t\t<tr>\n\t\t\t<td>\n\t\t\t<p>No. fields of view</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>MMC-</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>MMC+ (0.5 ug/ml 3h)</p>\n\t\t\t</td>\n\t\t</tr>\n\t\t<tr>\n\t\t\t<td>\n\t\t\t<p>RecA-mGFP</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>7</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>15</p>\n\t\t\t</td>\n\t\t</tr>\n\t\t<tr>\n\t\t\t<td>\n\t\t\t<p>RecB-sfGFP</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>17</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>21</p>\n\t\t\t</td>\n\t\t</tr>\n\t\t<tr>\n\t\t\t<td>\n\t\t\t<p>MG1655 control</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>10</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>10</p>\n\t\t\t</td>\n\t\t</tr>\n\t</tbody>\n</table>\n\n<p>Approx. size before/after compression: 21 GB / 7 GB</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "University of York", 
      "@id": "https://orcid.org/0000-0001-8802-352X", 
      "@type": "Person", 
      "name": "Alex L Payne-Dwyer"
    }, 
    {
      "affiliation": "University of York", 
      "@id": "https://orcid.org/0000-0003-3201-4135", 
      "@type": "Person", 
      "name": "Aisha H Syeda"
    }, 
    {
      "affiliation": "University of York", 
      "@id": "https://orcid.org/0000-0002-1715-1249", 
      "@type": "Person", 
      "name": "Mark C Leake"
    }
  ], 
  "url": "https://zenodo.org/record/6639101", 
  "datePublished": "2022-06-13", 
  "version": "1.0", 
  "keywords": [
    "DNA repair", 
    "single-molecule imaging", 
    "bacteria", 
    "Slimfield", 
    "fluorescence microscopy"
  ], 
  "@context": "https://schema.org/", 
  "distribution": [
    {
      "contentUrl": "https://zenodo.org/api/files/0f260a2a-5f34-40df-9f09-61ae424a539e/Open Data Proforma Slimfield E. coli repair proteins (RecA-mGFP and RecB-sfGFP).docx", 
      "encodingFormat": "docx", 
      "@type": "DataDownload"
    }, 
    {
      "contentUrl": "https://zenodo.org/api/files/0f260a2a-5f34-40df-9f09-61ae424a539e/RecAB raw Slimfield data.zip", 
      "encodingFormat": "zip", 
      "@type": "DataDownload"
    }
  ], 
  "identifier": "https://doi.org/10.5281/zenodo.6639101", 
  "@id": "https://doi.org/10.5281/zenodo.6639101", 
  "@type": "Dataset", 
  "name": "Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)"
}