June 13, 2022 Dataset Open Access

Alex L Payne-Dwyer; Aisha H Syeda; Mark C Leake

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        "title": "Pushing proteins off DNA - how do helicases unwind protein-coated DNA?", 
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    "references": [
      "\"RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live Escherichia coli with single-molecule sensitivity\", Alex L. Payne-Dwyer, Aisha H. Syeda, Jack W. Shepherd, Lewis Frame, Mark. C. Leake bioRxiv 2021.10.04.463068; doi: https://doi.org/10.1101/2021.10.04.463068"
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    "keywords": [
      "DNA repair", 
      "single-molecule imaging", 
      "bacteria", 
      "Slimfield", 
      "fluorescence microscopy"
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    "publication_date": "2022-06-13", 
    "creators": [
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        "orcid": "0000-0001-8802-352X", 
        "affiliation": "University of York", 
        "name": "Alex L Payne-Dwyer"
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      {
        "orcid": "0000-0003-3201-4135", 
        "affiliation": "University of York", 
        "name": "Aisha H Syeda"
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        "orcid": "0000-0002-1715-1249", 
        "affiliation": "University of York", 
        "name": "Mark C Leake"
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    "description": "<p>Imaging modality / instrument: <em>Brightfield</em> + <em>Slimfield</em></p>\n\n<p>Image format:<em> OME TIFF (16 bit) + MicroManager metadata files</em></p>\n\n<p>Microscope settings:</p>\n\n<p><em>488 nm triggered excitation; split detection, cropped to GFP or RFP/GFP (left/right) channels; 3 ms/frame laser exposure; Photometrics Prime95b CMOS</em></p>\n\n<p>Samples and acquisitions:</p>\n\n<p>Fluorescent fusions in live E.coli cells.&nbsp; MMC = mitomycin C</p>\n\n<table>\n\t<tbody>\n\t\t<tr>\n\t\t\t<td>\n\t\t\t<p>No. fields of view</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>MMC-</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>MMC+ (0.5 ug/ml 3h)</p>\n\t\t\t</td>\n\t\t</tr>\n\t\t<tr>\n\t\t\t<td>\n\t\t\t<p>RecA-mGFP</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>7</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>15</p>\n\t\t\t</td>\n\t\t</tr>\n\t\t<tr>\n\t\t\t<td>\n\t\t\t<p>RecB-sfGFP</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>17</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>21</p>\n\t\t\t</td>\n\t\t</tr>\n\t\t<tr>\n\t\t\t<td>\n\t\t\t<p>MG1655 control</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>10</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>10</p>\n\t\t\t</td>\n\t\t</tr>\n\t</tbody>\n</table>\n\n<p>Approx. size before/after compression: 21 GB / 7 GB</p>"
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