June 13, 2022 Dataset Open Access

Alex L Payne-Dwyer; Aisha H Syeda; Mark C Leake

DataCite XML Export

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  <identifier identifierType="DOI">10.5281/zenodo.6639101</identifier>
  <creators>
    <creator>
      <creatorName>Alex L Payne-Dwyer</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0001-8802-352X</nameIdentifier>
      <affiliation>University of York</affiliation>
    </creator>
    <creator>
      <creatorName>Aisha H Syeda</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-3201-4135</nameIdentifier>
      <affiliation>University of York</affiliation>
    </creator>
    <creator>
      <creatorName>Mark C Leake</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-1715-1249</nameIdentifier>
      <affiliation>University of York</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2022</publicationYear>
  <subjects>
    <subject>DNA repair</subject>
    <subject>single-molecule imaging</subject>
    <subject>bacteria</subject>
    <subject>Slimfield</subject>
    <subject>fluorescence microscopy</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2022-06-13</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Dataset"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/6639101</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.6639100</relatedIdentifier>
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  <version>1.0</version>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;Imaging modality / instrument: &lt;em&gt;Brightfield&lt;/em&gt; + &lt;em&gt;Slimfield&lt;/em&gt;&lt;/p&gt;

&lt;p&gt;Image format:&lt;em&gt; OME TIFF (16 bit) + MicroManager metadata files&lt;/em&gt;&lt;/p&gt;

&lt;p&gt;Microscope settings:&lt;/p&gt;

&lt;p&gt;&lt;em&gt;488 nm triggered excitation; split detection, cropped to GFP or RFP/GFP (left/right) channels; 3 ms/frame laser exposure; Photometrics Prime95b CMOS&lt;/em&gt;&lt;/p&gt;

&lt;p&gt;Samples and acquisitions:&lt;/p&gt;

&lt;p&gt;Fluorescent fusions in live E.coli cells.&amp;nbsp; MMC = mitomycin C&lt;/p&gt;

&lt;table&gt;
	&lt;tbody&gt;
		&lt;tr&gt;
			&lt;td&gt;
			&lt;p&gt;No. fields of view&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;MMC-&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;MMC+ (0.5 ug/ml 3h)&lt;/p&gt;
			&lt;/td&gt;
		&lt;/tr&gt;
		&lt;tr&gt;
			&lt;td&gt;
			&lt;p&gt;RecA-mGFP&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;7&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;15&lt;/p&gt;
			&lt;/td&gt;
		&lt;/tr&gt;
		&lt;tr&gt;
			&lt;td&gt;
			&lt;p&gt;RecB-sfGFP&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;17&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;21&lt;/p&gt;
			&lt;/td&gt;
		&lt;/tr&gt;
		&lt;tr&gt;
			&lt;td&gt;
			&lt;p&gt;MG1655 control&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;10&lt;/p&gt;
			&lt;/td&gt;
			&lt;td&gt;
			&lt;p&gt;10&lt;/p&gt;
			&lt;/td&gt;
		&lt;/tr&gt;
	&lt;/tbody&gt;
&lt;/table&gt;

&lt;p&gt;Approx. size before/after compression: 21 GB / 7 GB&lt;/p&gt;</description>
    <description descriptionType="Other">{"references": ["\"RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live Escherichia coli with single-molecule sensitivity\", Alex L. Payne-Dwyer, Aisha H. Syeda, Jack W. Shepherd, Lewis Frame, Mark. C. Leake bioRxiv 2021.10.04.463068; doi: https://doi.org/10.1101/2021.10.04.463068"]}</description>
  </descriptions>
  <fundingReferences>
    <fundingReference>
      <funderName>UK Research and Innovation</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/100014013</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/UKRI/BBSRC/BB/P000746/1/">BB/P000746/1</awardNumber>
      <awardTitle>Pushing proteins off DNA - how do helicases unwind protein-coated DNA?</awardTitle>
    </fundingReference>
    <fundingReference>
      <funderName>UK Research and Innovation</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/100014013</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/UKRI/BBSRC/BB/N006453/1/">BB/N006453/1</awardNumber>
      <awardTitle>Replication repair in real life: analysing how broken DNA replication machines are rebuilt inside cells.</awardTitle>
    </fundingReference>
    <fundingReference>
      <funderName>UK Research and Innovation</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/100014013</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/UKRI/EPSRC/EP/T002166/1/">EP/T002166/1</awardNumber>
      <awardTitle>Biological physics of protein clustering in epigenetic memory and transcriptional control</awardTitle>
    </fundingReference>
  </fundingReferences>
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