Dataset Open Access
Alex L Payne-Dwyer;
Aisha H Syeda;
Mark C Leake
<?xml version='1.0' encoding='utf-8'?> <rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:adms="http://www.w3.org/ns/adms#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dct="http://purl.org/dc/terms/" xmlns:dctype="http://purl.org/dc/dcmitype/" xmlns:dcat="http://www.w3.org/ns/dcat#" xmlns:duv="http://www.w3.org/ns/duv#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:frapo="http://purl.org/cerif/frapo/" xmlns:geo="http://www.w3.org/2003/01/geo/wgs84_pos#" xmlns:gsp="http://www.opengis.net/ont/geosparql#" xmlns:locn="http://www.w3.org/ns/locn#" xmlns:org="http://www.w3.org/ns/org#" xmlns:owl="http://www.w3.org/2002/07/owl#" xmlns:prov="http://www.w3.org/ns/prov#" xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#" xmlns:schema="http://schema.org/" xmlns:skos="http://www.w3.org/2004/02/skos/core#" xmlns:vcard="http://www.w3.org/2006/vcard/ns#" xmlns:wdrs="http://www.w3.org/2007/05/powder-s#"> <rdf:Description rdf:about="https://doi.org/10.5281/zenodo.6639101"> <rdf:type rdf:resource="http://www.w3.org/ns/dcat#Dataset"/> <dct:type rdf:resource="http://purl.org/dc/dcmitype/Dataset"/> <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#anyURI">https://doi.org/10.5281/zenodo.6639101</dct:identifier> <foaf:page rdf:resource="https://doi.org/10.5281/zenodo.6639101"/> <dct:creator> <rdf:Description rdf:about="http://orcid.org/0000-0001-8802-352X"> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#string">0000-0001-8802-352X</dct:identifier> <foaf:name>Alex L Payne-Dwyer</foaf:name> <org:memberOf> <foaf:Organization> <foaf:name>University of York</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description rdf:about="http://orcid.org/0000-0003-3201-4135"> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#string">0000-0003-3201-4135</dct:identifier> <foaf:name>Aisha H Syeda</foaf:name> <org:memberOf> <foaf:Organization> <foaf:name>University of York</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:creator> <rdf:Description rdf:about="http://orcid.org/0000-0002-1715-1249"> <rdf:type rdf:resource="http://xmlns.com/foaf/0.1/Agent"/> <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#string">0000-0002-1715-1249</dct:identifier> <foaf:name>Mark C Leake</foaf:name> <org:memberOf> <foaf:Organization> <foaf:name>University of York</foaf:name> </foaf:Organization> </org:memberOf> </rdf:Description> </dct:creator> <dct:title>Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)</dct:title> <dct:publisher> <foaf:Agent> <foaf:name>Zenodo</foaf:name> </foaf:Agent> </dct:publisher> <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#gYear">2022</dct:issued> <dcat:keyword>DNA repair</dcat:keyword> <dcat:keyword>single-molecule imaging</dcat:keyword> <dcat:keyword>bacteria</dcat:keyword> <dcat:keyword>Slimfield</dcat:keyword> <dcat:keyword>fluorescence microscopy</dcat:keyword> <frapo:isFundedBy rdf:resource="info:eu-repo/grantAgreement/UKRI/BBSRC/BB/P000746/1/"/> <schema:funder> <foaf:Organization> <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#string">10.13039/100014013</dct:identifier> <foaf:name>UK Research and Innovation</foaf:name> </foaf:Organization> </schema:funder> <frapo:isFundedBy rdf:resource="info:eu-repo/grantAgreement/UKRI/BBSRC/BB/N006453/1/"/> <schema:funder> <foaf:Organization> <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#string">10.13039/100014013</dct:identifier> <foaf:name>UK Research and Innovation</foaf:name> </foaf:Organization> </schema:funder> <frapo:isFundedBy rdf:resource="info:eu-repo/grantAgreement/UKRI/EPSRC/EP/T002166/1/"/> <schema:funder> <foaf:Organization> <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#string">10.13039/100014013</dct:identifier> <foaf:name>UK Research and Innovation</foaf:name> </foaf:Organization> </schema:funder> <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#date">2022-06-13</dct:issued> <dct:language rdf:resource="http://publications.europa.eu/resource/authority/language/ENG"/> <owl:sameAs rdf:resource="https://zenodo.org/record/6639101"/> <adms:identifier> <adms:Identifier> <skos:notation rdf:datatype="http://www.w3.org/2001/XMLSchema#anyURI">https://zenodo.org/record/6639101</skos:notation> <adms:schemeAgency>url</adms:schemeAgency> </adms:Identifier> </adms:identifier> <dct:isVersionOf rdf:resource="https://doi.org/10.5281/zenodo.6639100"/> <owl:versionInfo>1.0</owl:versionInfo> <dct:description><p>Imaging modality / instrument: <em>Brightfield</em> + <em>Slimfield</em></p> <p>Image format:<em> OME TIFF (16 bit) + MicroManager metadata files</em></p> <p>Microscope settings:</p> <p><em>488 nm triggered excitation; split detection, cropped to GFP or RFP/GFP (left/right) channels; 3 ms/frame laser exposure; Photometrics Prime95b CMOS</em></p> <p>Samples and acquisitions:</p> <p>Fluorescent fusions in live E.coli cells.&nbsp; MMC = mitomycin C</p> <table> <tbody> <tr> <td> <p>No. fields of view</p> </td> <td> <p>MMC-</p> </td> <td> <p>MMC+ (0.5 ug/ml 3h)</p> </td> </tr> <tr> <td> <p>RecA-mGFP</p> </td> <td> <p>7</p> </td> <td> <p>15</p> </td> </tr> <tr> <td> <p>RecB-sfGFP</p> </td> <td> <p>17</p> </td> <td> <p>21</p> </td> </tr> <tr> <td> <p>MG1655 control</p> </td> <td> <p>10</p> </td> <td> <p>10</p> </td> </tr> </tbody> </table> <p>Approx. size before/after compression: 21 GB / 7 GB</p></dct:description> <dct:description>{"references": ["\"RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live Escherichia coli with single-molecule sensitivity\", Alex L. Payne-Dwyer, Aisha H. Syeda, Jack W. Shepherd, Lewis Frame, Mark. C. Leake bioRxiv 2021.10.04.463068; doi: https://doi.org/10.1101/2021.10.04.463068"]}</dct:description> <dct:accessRights rdf:resource="http://publications.europa.eu/resource/authority/access-right/PUBLIC"/> <dct:accessRights> <dct:RightsStatement rdf:about="info:eu-repo/semantics/openAccess"> <rdfs:label>Open Access</rdfs:label> </dct:RightsStatement> </dct:accessRights> <dcat:distribution> <dcat:Distribution> <dct:license rdf:resource="https://creativecommons.org/licenses/by/4.0/legalcode"/> <dcat:accessURL rdf:resource="https://doi.org/10.5281/zenodo.6639101"/> </dcat:Distribution> </dcat:distribution> <dcat:distribution> <dcat:Distribution> <dcat:accessURL rdf:resource="https://doi.org/10.5281/zenodo.6639101"/> <dcat:byteSize>15827</dcat:byteSize> <dcat:downloadURL rdf:resource="https://zenodo.org/record/6639101/files/Open Data Proforma Slimfield E. coli repair proteins (RecA-mGFP and RecB-sfGFP).docx"/> <dcat:mediaType>application/vnd.openxmlformats-officedocument.wordprocessingml.document</dcat:mediaType> </dcat:Distribution> </dcat:distribution> <dcat:distribution> <dcat:Distribution> <dcat:accessURL rdf:resource="https://doi.org/10.5281/zenodo.6639101"/> <dcat:byteSize>7135725900</dcat:byteSize> <dcat:downloadURL rdf:resource="https://zenodo.org/record/6639101/files/RecAB raw Slimfield data.zip"/> <dcat:mediaType>application/zip</dcat:mediaType> </dcat:Distribution> </dcat:distribution> </rdf:Description> <foaf:Project rdf:about="info:eu-repo/grantAgreement/UKRI/BBSRC/BB/P000746/1/"> <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#string">BB/P000746/1</dct:identifier> <dct:title>Pushing proteins off DNA - how do helicases unwind protein-coated DNA?</dct:title> <frapo:isAwardedBy> <foaf:Organization> <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#string">10.13039/100014013</dct:identifier> <foaf:name>UK Research and Innovation</foaf:name> </foaf:Organization> </frapo:isAwardedBy> </foaf:Project> <foaf:Project rdf:about="info:eu-repo/grantAgreement/UKRI/BBSRC/BB/N006453/1/"> <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#string">BB/N006453/1</dct:identifier> <dct:title>Replication repair in real life: analysing how broken DNA replication machines are rebuilt inside cells.</dct:title> <frapo:isAwardedBy> <foaf:Organization> <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#string">10.13039/100014013</dct:identifier> <foaf:name>UK Research and Innovation</foaf:name> </foaf:Organization> </frapo:isAwardedBy> </foaf:Project> <foaf:Project rdf:about="info:eu-repo/grantAgreement/UKRI/EPSRC/EP/T002166/1/"> <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#string">EP/T002166/1</dct:identifier> <dct:title>Biological physics of protein clustering in epigenetic memory and transcriptional control</dct:title> <frapo:isAwardedBy> <foaf:Organization> <dct:identifier rdf:datatype="http://www.w3.org/2001/XMLSchema#string">10.13039/100014013</dct:identifier> <foaf:name>UK Research and Innovation</foaf:name> </foaf:Organization> </frapo:isAwardedBy> </foaf:Project> </rdf:RDF>
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