June 13, 2022 Dataset Open Access

Alex L Payne-Dwyer; Aisha H Syeda; Mark C Leake

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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.6639101", 
  "language": "eng", 
  "title": "Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)", 
  "issued": {
    "date-parts": [
      [
        2022, 
        6, 
        13
      ]
    ]
  }, 
  "abstract": "<p>Imaging modality / instrument: <em>Brightfield</em> + <em>Slimfield</em></p>\n\n<p>Image format:<em> OME TIFF (16 bit) + MicroManager metadata files</em></p>\n\n<p>Microscope settings:</p>\n\n<p><em>488 nm triggered excitation; split detection, cropped to GFP or RFP/GFP (left/right) channels; 3 ms/frame laser exposure; Photometrics Prime95b CMOS</em></p>\n\n<p>Samples and acquisitions:</p>\n\n<p>Fluorescent fusions in live E.coli cells.&nbsp; MMC = mitomycin C</p>\n\n<table>\n\t<tbody>\n\t\t<tr>\n\t\t\t<td>\n\t\t\t<p>No. fields of view</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>MMC-</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>MMC+ (0.5 ug/ml 3h)</p>\n\t\t\t</td>\n\t\t</tr>\n\t\t<tr>\n\t\t\t<td>\n\t\t\t<p>RecA-mGFP</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>7</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>15</p>\n\t\t\t</td>\n\t\t</tr>\n\t\t<tr>\n\t\t\t<td>\n\t\t\t<p>RecB-sfGFP</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>17</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>21</p>\n\t\t\t</td>\n\t\t</tr>\n\t\t<tr>\n\t\t\t<td>\n\t\t\t<p>MG1655 control</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>10</p>\n\t\t\t</td>\n\t\t\t<td>\n\t\t\t<p>10</p>\n\t\t\t</td>\n\t\t</tr>\n\t</tbody>\n</table>\n\n<p>Approx. size before/after compression: 21 GB / 7 GB</p>", 
  "author": [
    {
      "family": "Alex L Payne-Dwyer"
    }, 
    {
      "family": "Aisha H Syeda"
    }, 
    {
      "family": "Mark C Leake"
    }
  ], 
  "version": "1.0", 
  "type": "dataset", 
  "id": "6639101"
}