Dataset Open Access

Shedding Light on Metal-Based Nanoparticles in Zebrafish by Computed Tomography with Micrometer Resolution

HUWYLER Jörg


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    <subfield code="a">HUWYLER Jörg</subfield>
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    <subfield code="a">Shedding Light on Metal-Based Nanoparticles in Zebrafish by Computed Tomography with Micrometer Resolution</subfield>
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    <subfield code="a">&lt;p&gt;Supplementary 3D image stacks of microtomography data.&lt;/p&gt;

&lt;p&gt;100 layer xy, xz, and yz image stacks&lt;/p&gt;

&lt;p&gt;Publication included as PDF file (open access, DOI: 10.1002/smll.202000746)&lt;/p&gt;

&lt;p&gt;********************************************&lt;/p&gt;

&lt;p&gt;Metal-based nanoparticles are clinically used for diagnostic and therapeutic&lt;br&gt;
applications. After parenteral administration, they will distribute throughout&lt;br&gt;
different organs. Quantification of their distribution within tissues in the 3D&lt;br&gt;
space, however, remains a challenge owing to the small particle diameter.&lt;br&gt;
In this study, synchrotron radiation-based hard X-ray tomography (SR&amp;mu;CT)&lt;br&gt;
in absorption and phase contrast modes is evaluated for the localization of&lt;br&gt;
superparamagnetic iron oxide nanoparticles (SPIONs) in soft tissues based&lt;br&gt;
on their electron density and X-ray attenuation. Biodistribution of SPIONs&lt;br&gt;
is studied using zebrafish embryos as a vertebrate screening model. This&lt;br&gt;
label-free approach gives rise to an isotropic, 3D, direct space visualization&lt;br&gt;
of the entire 2.5 mm-long animal with a spatial resolution of around 2&lt;br&gt;
&amp;mu;m. High resolution image stacks are available on a dedicated internet&lt;br&gt;
page (http://zebrafish.pharma-te.ch). X-ray tomography is combined with&lt;br&gt;
physico-chemical characterization and cellular uptake studies to confirm the&lt;br&gt;
safety and effectiveness of protective SPION coatings. It is demonstrated&lt;br&gt;
that SR&amp;mu;CT provides unprecedented insights into the zebrafish embryo&lt;br&gt;
anatomy and tissue distribution of label-free metal oxide nanoparticles.&lt;/p&gt;</subfield>
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