Chen, Gregory M
2022-02-02
<p>CITE-Seq and CyTOF data and code: CAR T cells from two patients with long-term CLL remission.</p>
<p>This is the accompanying dataset for the manuscript:</p>
<p>Melenhorst JJ, Chen GM, Wang M, Porter DL, et al. Decade-long leukaemia remissions with persistence of CD4+ CAR T cells. Nature, 2022. PMID: 35110735</p>
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<p>Manuscript abstract:</p>
<p>The adoptive transfer of T lymphocytes reprogrammed to target tumour cells has demonstrated potential for treatment of various cancers. However, little is known about the long-term potential and clonal stability of the infused cells. Here we studied long-lasting CD19-redirected chimeric antigen receptor (CAR) T cells in two patients with chronic lymphocytic leukaemia who achieved a complete remission in 2010. CAR T cells remained detectable more than ten years after infusion, with sustained remission in both patients. Notably, a highly activated CD4+ population emerged in both patients, dominating the CAR T cell population at the later time points. This transition was reflected in the stabilization of the clonal make-up of CAR T cells with a repertoire dominated by a small number of clones. Single-cell profiling demonstrated that these long-persisting CD4+ CAR T cells exhibited cytotoxic characteristics along with ongoing functional activation and proliferation. In addition, longitudinal profiling revealed a population of gamma delta CAR T cells that prominently expanded in one patient concomitant with CD8+ CAR T cells during the initial response phase. Our identification and characterization of these unexpected CAR T cell populations provide novel insight into the CAR T cell characteristics associated with anti-cancer response and long-term remission in leukaemia.</p>
https://doi.org/10.5281/zenodo.6567602
oai:zenodo.org:6567602
Zenodo
https://zenodo.org/communities/single_cell_rna_seq_analysis
https://doi.org/10.5281/zenodo.6567601
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Data and code from: Decade-long leukaemia remissions with persistence of CD4+ CAR Tcells
info:eu-repo/semantics/other