EXTRACTION, PHYTOCHEMICAL INVESTIGATION AND ANTIULCER ACTIVITY OF HYDROALCOHOLIC EXTRACT OF MIRABILIS LONGIFLORA

Gastroesophageal reflux disease, gastritis, peptic ulcer, duodenal ulcer, and other peptic illnesses are frequent in today's lifestyle. This could be the result of a hectic lifestyle or an unbalanced diet. The pathophysiology underlying these illnesses could be a mismatch between offensive and defensive mechanisms, either due to excessive acid and pepsin secretion or a decreased ability of the gastro-duodenal mucosal barrier to guard against acid-pepsin secretion from the stomach. Non-steroidal anti-inflammatory drugs (NSAIDs) are a type of therapy that has been shown to be beneficial in treating a variety of ailments. Some people take NSAIDs every day as a preventative measure. NSAIDs, on the other hand, can cause a wide range of serious adverse effects. Natural compounds that are edible have been found in studies to help prevent stomach ulcers. The goal of this study was to see if a hydroalcoholic extract of Mirabilis longiflora rhizomes has antiulcer efficacy in rats. The well-known test methodology was used to determine qualitative analysis of various phytochemical elements as well as quantitative analysis of total flavonoids and alkaloids. The anti-ulcer effect of hydroalcoholic extract in rats was tested in vivo against an ethanol-induced stomach ulcer. The volume and pH of stomach fluid, free acidity, total acidity, pepsin activity, ulcer index, and percent inhibition of ulcer index were all used as outcome measures, depending on the model. Phenols, flavonoids, saponins, and tannins were found in preliminary phytochemical screening. Total flavonoids (0.438 mg/100mg) were found in Mirabilis longiflora extract, followed by alkaloids (0.896 mg/100mgFurther hydroalcoholic extracts of 100 and 200 mg/kg/p.o dramatically reduced the volume of gastric content, total/free acidity, and pepsin activity, while increasing the pH of the gastric juice. The results of this investigation indicated that Mirabilis longiflora extract had anti-ulcer pharmacologic activity as a result of one or more secondary metabolites found in it. As a result, this research backs up its anti-ulcer use in Indian folk medicine. More research is needed into isolating particular phytochemicals and understanding mechanisms of action.

Keywords: Mirabilis longiflora, Phytochemical constituents, Antiulcer, Non-steroidal anti-inflammatory drugs, Ethanol-induced gastric ulcer.