Medical ozone modifies D-dimer, interleukin-6, lactic acid and oxidative stress levels: A possibility for the comprehensive treatment of COVID-19
Creators
- 1. Centro Médico Nacional de Biología Molecular. Puebla 72140, México; Instituto de Estudios Superiores en Biotecnología Médica. Puebla 75750, México; Sociedad Médica de Ozonoterapia de México, A.C.
- 2. Centro Médico Nacional de Biología Molecular. Puebla 72140, México; Instituto de Estudios Superiores en Biotecnología Médica. Puebla 75750, México
- 3. Instituto de Estudios Superiores en Biotecnología Médica. Puebla 75750, México; Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla. Puebla 72570, México.
- 4. Facultad de Medicina, Benemérita Universidad Autónoma de Puebla. Puebla 72570, México.
Description
ABSTRACT
Background: SARS-CoV-2-induced inflammation in COVID-19 is mediated by cytotoxic and pro-oxidant effects that potentiate alveolar, endothelial and immune tissue damage. Objective: We investigated the effect of medicinal ozone administration on the oxidative stress markers; in addition to D-dimer, lactic acid and interleukin-6 as markers of endothelial injury and inflammation process. Methodology: Medicinal ozone with oligo metals was administered in vivo (major autohemotherapy) and in vitro (peripheral blood), to subsequently determine the levels of: H2O2, NO, GPx, CAT, TAP, TBARs, D-dimer, lactic acid and interleukin-6. Results: Medicinal ozone administration with oligo metals induced changes in oxidative stress markers both in vitro and in vivo. The H2O2 and TBARs levels decreased, in turn, NO levels increased (cardiovascular function marker). On the other hand, the levels of the antioxidant enzymes (GPx and CAT) show slightly increase, which indicates an antioxidant enzyme system regulation that counteracts the pro-oxidative effect of the infection. Furthermore, interleukin-6 levels decreased indicating the regulation of the systemic inflammatory process. Finally, lactic acid and D-dimer levels were decreased, establishing an improvement of energy metabolism and endothelial function respectively. Conclusion: The medicinal ozone administration induce decrease in the markers levels of oxidative stress, inflammation and cellular damage, improving the enzymatic antioxidant capacity and cellular metabolism with decrease plaque aggregation that contribute to reducing the risk of vascular endothelial damage. These benefits could be feasible to integrate in the treatment of endothelial injury in COVID-19 patients.
RESUMEN
Antecedentes: La inflamación inducida por SARS-CoV-2 en COVID-19 está mediada por efectos citotóxicos y pro-oxidativos que potencializan el daño del tejido alveolar, endotelial e inmunitario. Objetivo: Investigamos el efecto de la administración de ozono medicinal sobre marcadores de estrés oxidativo; además del dímero-D, ácido láctico e interleucina-6 como marcadores del proceso de lesión endotelial e inflamación. Metodología: Se administró ozono medicinal con oligometales in vivo (autohemoterapia mayor) e in vitro (sangre periférica), para determinar los niveles de: H2O2, NO, GPx, CAT, TAP, TBARs, dímero-D, ácido láctico e interleucina-6. Resultados: La administración de ozono medicinal con oligometales indujo cambios en los marcadores de estrés oxidativo tanto in vitro como in vivo. Los niveles de H2O2 y TBARs disminuyeron, a su vez, los niveles de NO aumentaron (marcador de función cardiovascular); además los niveles de enzimas antioxidantes (GPx y CAT) mostraron un leve aumento, indicando una posible regulación del sistema antioxidante enzimático que contrarresta el efecto pro-oxidativo de la infección. Además, los niveles de interleucina-6 disminuyeron indicando la regulación del proceso inflamatorio sistémico. Finalmente, se redujeron los niveles de ácido láctico y dímero-D, estableciendo una mejora del metabolismo energético y de la función endotelial respectivamente. Conclusión: La administración de ozono medicinal induce la disminución en los niveles de marcadores de estrés oxidativo, inflamación y daño celular, mejorando la capacidad antioxidante enzimática y el metabolismo celular con disminución de la agregación plaquetaria que contribuyen a reducir el riesgo del daño endotelial vascular. Estos beneficios podrían ser factibles de integrar en el tratamiento de las complicaciones vasculares y de la lesión endotelial en pacientes con COVID-19.
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- Journal article: https://www.aytbuap.mx/aytbuap-726/medical-ozone-modifies-d-dimer-interleukin-6-lactic-acid-and-oxidative-st (URL)
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