Technical note Open Access

Production of Recombinant α Synuclein Monomers and Preformed Fibrils (PFFs)

Maneca, Destiny-Love; Luo, Wen; Krahn, Andrea; Del Cid Pellitero, Esther; Shlaifer, Irina; Nicouleau, Michael; Pimentel, Luisa; Beitel, Lenore K.; Rao, Trisha; Durcan, Thomas M.

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  <dc:creator>Maneca, Destiny-Love</dc:creator>
  <dc:creator>Luo, Wen</dc:creator>
  <dc:creator>Krahn, Andrea</dc:creator>
  <dc:creator>Del Cid Pellitero, Esther</dc:creator>
  <dc:creator>Shlaifer, Irina</dc:creator>
  <dc:creator>Nicouleau, Michael</dc:creator>
  <dc:creator>Pimentel, Luisa</dc:creator>
  <dc:creator>Beitel, Lenore K.</dc:creator>
  <dc:creator>Rao, Trisha</dc:creator>
  <dc:creator>Durcan, Thomas M.</dc:creator>
  <dc:date>2022-04-09</dc:date>
  <dc:description>This protocol describes how to:
• Produce and purify recombinant human and mouse α-synuclein in monomeric form from transformed bacteria
• Generate α-synuclein preformed fibrils (PFFs) from recombinant monomeric α-synuclein
• Fluorescently label α-synuclein PFFs

Version 3.0 now available</dc:description>
  <dc:identifier>https://zenodo.org/record/6430401</dc:identifier>
  <dc:identifier>10.5281/zenodo.6430401</dc:identifier>
  <dc:identifier>oai:zenodo.org:6430401</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>doi:10.5281/zenodo.3372887</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/sgc-opennotebook</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>https://creativecommons.org/licenses/by/4.0/legalcode</dc:rights>
  <dc:subject>alpha-synuclein</dc:subject>
  <dc:subject>protein expression</dc:subject>
  <dc:subject>bacteria</dc:subject>
  <dc:subject>GST-tag</dc:subject>
  <dc:subject>Preformed fibrils (PFFs)</dc:subject>
  <dc:title>Production of Recombinant α Synuclein Monomers and Preformed Fibrils (PFFs)</dc:title>
  <dc:type>info:eu-repo/semantics/technicalDocumentation</dc:type>
  <dc:type>publication-technicalnote</dc:type>
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Publication date:
April 9, 2022
DOI:
10.5281/zenodo.6430401

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Keyword(s):
alpha-synuclein protein expression bacteria GST-tag Preformed fibrils (PFFs)
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Creative Commons Attribution 4.0 International

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Version V3.0 10.5281/zenodo.6430401 Apr 9, 2022
Version V2.0 10.5281/zenodo.3738335 Aug 21, 2019
Version V1.0 10.5281/zenodo.3372888 Aug 21, 2019
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