Maneca, Destiny-Love
Luo, Wen
Krahn, Andrea
Del Cid Pellitero, Esther
Shlaifer, Irina
Nicouleau, Michael
Pimentel, Luisa
Beitel, Lenore K.
Rao, Trisha
Durcan, Thomas M.
2022-04-09
<p>This protocol describes how to:<br>
• Produce and purify recombinant human and mouse α-synuclein in monomeric form from transformed bacteria<br>
• Generate α-synuclein preformed fibrils (PFFs) from recombinant monomeric α-synuclein<br>
• Fluorescently label α-synuclein PFFs</p>
<p>Version 3.0 now available</p>
https://doi.org/10.5281/zenodo.6430401
oai:zenodo.org:6430401
eng
Zenodo
https://zenodo.org/communities/sgc-opennotebook
https://doi.org/10.5281/zenodo.3372887
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
alpha-synuclein
protein expression
bacteria
GST-tag
Preformed fibrils (PFFs)
Production of Recombinant α Synuclein Monomers and Preformed Fibrils (PFFs)
info:eu-repo/semantics/technicalDocumentation