Technical note Open Access

Production of Recombinant α Synuclein Monomers and Preformed Fibrils (PFFs)

Maneca, Destiny-Love; Luo, Wen; Krahn, Andrea; Del Cid Pellitero, Esther; Shlaifer, Irina; Nicouleau, Michael; Pimentel, Luisa; Beitel, Lenore K.; Rao, Trisha; Durcan, Thomas M.


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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.6430401", 
  "language": "eng", 
  "title": "Production of Recombinant \u03b1 Synuclein Monomers and Preformed Fibrils (PFFs)", 
  "issued": {
    "date-parts": [
      [
        2022, 
        4, 
        9
      ]
    ]
  }, 
  "abstract": "<p>This protocol describes how to:<br>\n&bull; Produce and purify recombinant human and mouse &alpha;-synuclein in monomeric form from transformed bacteria<br>\n&bull; Generate &alpha;-synuclein preformed fibrils (PFFs) from recombinant monomeric &alpha;-synuclein<br>\n&bull; Fluorescently label &alpha;-synuclein PFFs</p>\n\n<p>Version 3.0 now available</p>", 
  "author": [
    {
      "family": "Maneca, Destiny-Love"
    }, 
    {
      "family": "Luo, Wen"
    }, 
    {
      "family": "Krahn, Andrea"
    }, 
    {
      "family": "Del Cid Pellitero, Esther"
    }, 
    {
      "family": "Shlaifer, Irina"
    }, 
    {
      "family": "Nicouleau, Michael"
    }, 
    {
      "family": "Pimentel, Luisa"
    }, 
    {
      "family": "Beitel, Lenore K."
    }, 
    {
      "family": "Rao, Trisha"
    }, 
    {
      "family": "Durcan, Thomas M."
    }
  ], 
  "version": "V3.0", 
  "type": "article", 
  "id": "6430401"
}
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