EVALUATION OF ANTIOXIDANT, ANTIDIABETIC AND ANTIHYPERLIPIDAEMIC ACTIVITY OF METHANOLIC EXTRACT OF CASEARIA ELLIPTICA IN ALLOXAN INDUCED MALE WISTAR DIABETIC RATS

Background: Diabetes mellitus is a group of metabolic disorders with the common manifestations, hyperglycemia.. Alloxan causes a massive reduction in insulin release by the destruction of β cells of the islets of langerhans, there by inducing hyperglycaemia. Alloxan has been shown to induce free radical production and cause tissue injury. The aim of the present investigation was to evaluate the antioxidant, antihyperlipidemic and antidiabetic activity of methanolic extract taken from Casearia elliptica.

Methods: The methanolic  extract of Casearia elliptica  whole plant used for the study. The Phytochemical test, acute toxicity study and oral glucose test was performed. Diabetes was induced in rat by single intra-peritoneal injection of alloxan (120 mg/kg). Male albino Wistar rats were divided into five groups each consisting of six animals as follows:Group I- Administered vehicle serves as Normal control.,Group II- Administered Alloxan (120 mg/kg sc) serves as diabetic control,Group III- Administered Reference Standard, (Glibenclamide 10 mg/kg, orally once daily),Group IV- Diabetic rats treated with Casearia elliptica (250mg/kg b.wt), serves as treated group,Group V- Diabetic rats treated with Casearia elliptica (500mg/kg b.wt), serves as treated group.. Bodyweight of each rat in the different groups was recorded daily. Biochemical and antioxidant enzyme parameters were determined on day 14. Histology of different organ (heart, liver, kidney, and pancreas) was performed after sacrificing the rats with euthanasia.

Results: Preliminary Phytochemical investigation of methanolic extracts of whole plant of Casearia elliptica was carried out and found that it contains fixed oil, saponins, alkaloids and carbohydrates. The maximum tolerated dose of root of Casearia elliptica was found as 2000 mg/kg after completion of toxicity study according to OECD guideline. The methanolic extract of whole plant of Casearia elliptica has potential anti-diabetic action in alloxan  induced diabetic rats and the effect was found equally effective with glibenclamide at higher dose. Our study clearly reveals that MECE has some obvious therapeutic implications in treating hyperglycemia, atherogenic lipoprotein profile and also possess antioxidant activity

Keywords: Diabetes mellitus, Alloxan, Casearia elliptica, Serum Triglycerides,