PHYTOCHEMICAL SCREENING AND DEVELOPMENT OF PHYTOSOMES OF HYDROALCOHOLIC EXTRACT OF WRIGHTIA TINCTORIA (ROXB.) R. BR FOR EFFECTIVE TREATMENT OF HEPATIC DISEASE

Wrightia tinctoria (Roxb.) R.Br. (W. tinctoria) has been reported to exhibit number of therapeutic uses such as astringent, stomachic, febrifuge, skin diseases and tonic in India. In the current days, most of the prevailing diseases and nutritional disorders are treated with natural medicines. The effectiveness of any herbal medication is dependent on the delivery of effective level of the therapeutically active compound. But a severe limitation exists in their bioavailability when administered orally or by topical applications. Phytosomes are recently introduced herbal formulations that are better absorbed and as a result produced better bioavailability and actions than the conventional phyto molecules or botanical extracts. The aim of the present study was to evaluate qualitative and quantitative phytochemical analysis, formulation and evaluation of phytosomes of hydroalcoholic leaf extract of W. tinctoria. The phytosome was prepared by the hydroalcoholic extract of plant and phospholipids: cholesterol by simple method. Characterization of phytosome was done by FTIR, entrapment efficiency, particle size and size distribution, optical microscopic study, stability studies and In vitro dissolution studies. Phytochemical analysis revealed the presence of alkaloids, glycosides, flavonoids, proteins, carbohydrates and saponins. The total flavonoids and alkaloid content of hydroalcoholic leaves extract of W. tinctoria was found to be 0.752 and 0.489mg/100mg respectively. Particle size and entrapment efficiency of optimized batch F3 was founds to be196.65±0.36nm and73.32±0.14. Combination of phospholipids and W. tinctoria can result in synergistic effect, For the future purpose it can used as a targeting drug delivery system as a liver targeting, brain targeting, cardio protective etc. Novel approach for herbal drug delivery is more prominent than conventional which improves bioavailability of polar extract and also patient compliance.

Keywords: Wrightia tinctoria, Phytosome, Phospholipids, Phytochemical analysis, Characterization