Dataset Open Access

Training material for RNA sequencing

Grüning, Björn; Erxleben, Anika; Houwaart, Torsten; Batut, Bérénice


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    <subfield code="a">&lt;p&gt;&lt;strong&gt;RNA-seq&lt;/strong&gt; (&lt;strong&gt;RNA sequencing&lt;/strong&gt;) uses high-throughput (HTS) data to reveal the presence and quantity of RNA in a biological sample at a given moment in time.&lt;/p&gt;

&lt;p&gt;RNA-Seq is used to analyze the continually changing cellular transcriptome. Specifically, RNA-Seq facilitates the ability to look at alternative gene spliced transcripts, post-transcriptional modifications, gene fusion, mutations/SNPs and changes in gene expression.&lt;sup&gt;[4]&lt;/sup&gt; In addition to mRNA transcripts, RNA-Seq can look at different populations of RNA to include total RNA, small RNA, such as miRNA, tRNA, and ribosomal profiling.&lt;/p&gt;</subfield>
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