labwares: # Pipettes ############################################# # Opentrons P20 Single-Channel Electronic Pipette p20_single: id: p20_single_gen2 # Opentrons P300 8-Channel Electronic Pipette p300_multi: id: p300_multi_gen2 # Modules ############################################### # Opentrons magnetic module mag_deck: # id: magdeck id: magnetic module gen2 # BRS script 2 # Tip racks ############################################# # Opentrons 20μL tips rack 96_tiprack_20ul: # id: opentrons_96_tiprack_20ul id: tipone_3dprinted_96_tiprack_20ul # BRS # Opentrons 300μL tips rack 96_tiprack_300ul: # id: opentrons_96_tiprack_300ul id: tipone_yellow_3dprinted_96_tiprack_300ul # BRS # Plates ################################################ # Opentrons 4-in-1 tubes rack for 1.5 ml eppendorf tubes 24_tuberack_1500ul: # id: e14151500starlab_24_tuberack_1500ul id: opentrons_24_tuberack_eppendorf_1.5ml_safelock_snapcap # BRS scripts 1, 3, 4 # 96 well rigid PCR plate (clip and transformation steps) 96_wellplate_200ul_pcr_step_14: # id: 4ti0960rig_96_wellplate_200ul id: green_96_wellplate_200ul_pcr # BRS scripts 1, 4 # 96 well rigid PCR plate (purification and assembly steps) 96_wellplate_200ul_pcr_step_23: # id: 4ti0960rig_96_wellplate_200ul id: black_96_wellplate_200ul_pcr # BRS script 2, 3 # Agar plate (transformation step) agar_plate_step_4: # id: 4ti0960rig_96_wellplate_200ul id: thermoomnitrayfor96spots_96_wellplate_50ul # BRS script 4 # Reservoir plate 21 mL 12 channels 12_reservoir_21000ul: # id: 4ti0131_12_reservoir_21000ul id: citadel_12_wellplate_22000ul # BRS script 2 # 96 deep well plate 2 mL wells 96_deepwellplate_2ml: # id: 4ti0136_96_wellplate_2200ul id: transparent_96_wellplate_2ml_deep # BRS scripts 2, 4 # Corning 12 Well Plate 6.9 mL Flat 12_corning_wellplate: id: corning_12_wellplate_6.9ml_flat parameters: # Purification step ##################################### # Magnetic module height (mm) - purification step purif_magdeck_height: # value: 20 value: 10.8 # BRS # Washing time (min) - purification step purif_wash_time: value: 0.5 # Bead ratio - purification step purif_bead_ratio: value: 1.8 # Incubation time (min) - purification step purif_incubation_time: value: 5 # Settling time (min) - purification step purif_settling_time: # value: 2 value: 6 # BRS # Drying time (min) - purification step purif_drying_time: # value: 5 value: 15 # BRS # Elution time (min) - purif step purif_elution_time: # value: 2 value: 5 # BRS # Transformation step ################################### # Incubation temperature transfo_incubation_temp: # value: 4 value: 8 # BRS # Incubation time (min) transfo_incubation_time: # value: 20 value: 30 # BRS