Journal article Open Access

Find and cut-and-transfer (FiCAT) mammalian genome engineering

Pallarès-Masmitjà, Maria; Ivančić, Dimitrije; Mir-Pedrol, Júlia; Jaraba-Wallace, Jessica; Tagliani, Tommaso; Oliva, Baldomero; Rahmeh, Amal; Sánchez-Mejías, Avencia; Güell, Marc


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{
  "description": "<p><strong>Abstract</strong></p>\n\n<p>While multiple technologies for small allele genome editing exist, robust technologies for targeted integration of large DNA fragments in mammalian genomes are still missing. Here we develop a gene delivery tool (FiCAT) combining the precision of a CRISPR-Cas9 (find module), and the payload transfer efficiency of an engineered piggyBac transposase (cut-and-transfer module). FiCAT combines the functionality of Cas9 DNA scanning and targeting DNA, with piggyBac donor DNA processing and transfer capacity. PiggyBac functional domains are engineered providing increased on-target integration while reducing off-target events. We demonstrate efficient delivery and programmable insertion of small and large payloads in cellulo (human (Hek293T, K-562) and mouse (C2C12)) and in vivo in mouse liver. Finally, we evolve more efficient versions of FiCAT by generating a targeted diversity of 394,000 variants and undergoing 4 rounds of evolution. In this work, we develop a precise and efficient targeted insertion of multi kilobase DNA fragments in mammalian genomes.</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Department of Health and Experimental Sciences, Pompeu Fabra University, Barcelona, Spain", 
      "@id": "https://orcid.org/0000-0002-7317-7793", 
      "@type": "Person", 
      "name": "Pallar\u00e8s-Masmitj\u00e0,\u00a0Maria"
    }, 
    {
      "affiliation": "Department of Health and Experimental Sciences, Pompeu Fabra University, Barcelona, Spain - Barcelona Institute of Science and Technology, Barcelona, Spain", 
      "@type": "Person", 
      "name": "Ivan\u010di\u0107,\u00a0Dimitrije"
    }, 
    {
      "affiliation": "Department of Health and Experimental Sciences, Pompeu Fabra University, Barcelona, Spain", 
      "@id": "https://orcid.org/0000-0001-6104-9260", 
      "@type": "Person", 
      "name": "Mir-Pedrol,\u00a0J\u00falia"
    }, 
    {
      "affiliation": "Department of Health and Experimental Sciences, Pompeu Fabra University, Barcelona, Spain", 
      "@type": "Person", 
      "name": "Jaraba-Wallace,\u00a0Jessica"
    }, 
    {
      "affiliation": "Department of Health and Experimental Sciences, Pompeu Fabra University, Barcelona, Spain", 
      "@type": "Person", 
      "name": "Tagliani,\u00a0Tommaso"
    }, 
    {
      "affiliation": "Department of Health and Experimental Sciences, Pompeu Fabra University, Barcelona, Spain", 
      "@id": "https://orcid.org/0000-0003-0702-0250", 
      "@type": "Person", 
      "name": "Oliva,\u00a0Baldomero"
    }, 
    {
      "affiliation": "Department of Health and Experimental Sciences, Pompeu Fabra University, Barcelona, Spain", 
      "@type": "Person", 
      "name": "Rahmeh,\u00a0Amal"
    }, 
    {
      "affiliation": "Department of Health and Experimental Sciences, Pompeu Fabra University, Barcelona, Spain", 
      "@type": "Person", 
      "name": "S\u00e1nchez-Mej\u00edas,\u00a0Avencia"
    }, 
    {
      "affiliation": "Department of Health and Experimental Sciences, Pompeu Fabra University, Barcelona, Spain", 
      "@id": "https://orcid.org/0000-0003-4000-7912", 
      "@type": "Person", 
      "name": "G\u00fcell,\u00a0Marc"
    }
  ], 
  "headline": "Find and cut-and-transfer (FiCAT) mammalian genome engineering", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2021-12-03", 
  "url": "https://zenodo.org/record/6104372", 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.1038/s41467-021-27183-x", 
  "@id": "https://doi.org/10.1038/s41467-021-27183-x", 
  "@type": "ScholarlyArticle", 
  "name": "Find and cut-and-transfer (FiCAT) mammalian genome engineering"
}
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