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Published January 31, 2022 | Version 2.2.0
Software Open

nf-core/ampliseq: Ampliseq Version 2.2.0

  • 1. QBiC
  • 2. Boehringer Ingelheim
  • 3. Linnaeus University & Stockholm University
  • 4. National Bioinformatics Infrastructure Sweden (NBIS)
  • 5. HUJI
  • 6. @qbicsoftware
  • 7. @SciLifeLab | Karolinska Institutet
  • 8. Science for Life Laboratory
  • 9. Seqera Labs

Description

nf-core/ampliseq version 2.2.0 - 2022-01-31 Summary of changes

  • Added features (e.g. SBDI-GTDB db update, Adonis test)
  • Added convenience updates (e.g. properly reporting and on demand ignoring low read samples)
  • Updated to nf-core template 2.2
  • Fixed some bugs
Detailed changes Added
  • #352, #372 - --skip_dada_addspecies allows to skip species level classification to reduce memory requirements, incompatible with --sbdiexport that expect species annotation.
  • #364 - Adonis in QIIME2 for testing feature importance in beta diversity distances, --qiime_adonis_formula can be set to provide a custom formula.
  • #366 - New version of the SBDI-GTDB taxonomy database: v. 3. (Fixes problem with Reverse_ added to some domain strings.)
Changed
  • #354 - Input files and files after primer trimming with cutadapt are required to be >1KB (i.e. not empty) and either the pipeline will stop if at least one sample file fails or the failing samples will be ignored when using --ignore_empty_input_files or --ignore_failed_trimming, respectively.
  • #376 - Forbid sampleIDs starting with a number when also --metadata is used, because such strings are unintentionally modified and the metadata will not match any more.
Fixed
  • #377- An error message will occur when --sbdiexport is used with --skip_taxonomy or --skip_dada_addspecies
  • #375- Updated documentation regarding not using curly brackets in --extension with --single_end
  • #362- Template update for nf-core/tools version 2.2, now requires nextflow version >= 21.10.3
  • #374- Cutadapt results can be now also viwed in the MultiQC report
Dependencies
Tool Previous version New version
Cutadapt 3.2 3.4
DADA2 1.20.0 1.22.0
QIIME2 2021.2 2021.8
PICRUSt2 2.4.1 2.4.2
MultiQC 1.10.1 1.11
Removed
  • #350 - Remove redundant derepFastq step (has no impact on results)

Files

nf-core/ampliseq-2.2.0.zip

Files (3.4 MB)

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Additional details

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