Published January 31, 2022
| Version 2.2.0
Software
Open
nf-core/ampliseq: Ampliseq Version 2.2.0
Creators
- 1. QBiC
- 2. Boehringer Ingelheim
- 3. Linnaeus University & Stockholm University
- 4. National Bioinformatics Infrastructure Sweden (NBIS)
- 5. HUJI
- 6. @qbicsoftware
- 7. @SciLifeLab | Karolinska Institutet
- 8. Science for Life Laboratory
- 9. Seqera Labs
Description
nf-core/ampliseq version 2.2.0 - 2022-01-31 Summary of changes
- Added features (e.g. SBDI-GTDB db update, Adonis test)
- Added convenience updates (e.g. properly reporting and on demand ignoring low read samples)
- Updated to nf-core template 2.2
- Fixed some bugs
Added
- #352, #372 -
--skip_dada_addspecies
allows to skip species level classification to reduce memory requirements, incompatible with--sbdiexport
that expect species annotation. - #364 - Adonis in QIIME2 for testing feature importance in beta diversity distances,
--qiime_adonis_formula
can be set to provide a custom formula. - #366 - New version of the SBDI-GTDB taxonomy database: v. 3. (Fixes problem with
Reverse_
added to some domain strings.)
Changed
- #354 - Input files and files after primer trimming with cutadapt are required to be >1KB (i.e. not empty) and either the pipeline will stop if at least one sample file fails or the failing samples will be ignored when using
--ignore_empty_input_files
or--ignore_failed_trimming
, respectively. - #376 - Forbid sampleIDs starting with a number when also
--metadata
is used, because such strings are unintentionally modified and the metadata will not match any more.
Fixed
- #377- An error message will occur when
--sbdiexport
is used with--skip_taxonomy
or--skip_dada_addspecies
- #375- Updated documentation regarding not using curly brackets in
--extension
with--single_end
- #362- Template update for nf-core/tools version 2.2, now requires nextflow version
>= 21.10.3
- #374- Cutadapt results can be now also viwed in the MultiQC report
Dependencies
Tool | Previous version | New version |
---|---|---|
Cutadapt | 3.2 | 3.4 |
DADA2 | 1.20.0 | 1.22.0 |
QIIME2 | 2021.2 | 2021.8 |
PICRUSt2 | 2.4.1 | 2.4.2 |
MultiQC | 1.10.1 | 1.11 |
Removed
- #350 - Remove redundant derepFastq step (has no impact on results)
Files
nf-core/ampliseq-2.2.0.zip
Files
(3.4 MB)
Name | Size | Download all |
---|---|---|
md5:82e2070f5b84c7d2556150ab38c99789
|
3.4 MB | Preview Download |
Additional details
Related works
- Is supplement to
- https://github.com/nf-core/ampliseq/tree/2.2.0 (URL)