Report Open Access
Interpretative toolkit to enable the use of genomic data aligned with physiological status for representative mixoplankton.
ANESTIS Konstantinos;
MANSOUR Joost Samir;
MASELLI Maira;
HANSEN Per Juel;
NOT Fabrice;
JOHN Uwe
Executive Summary
In Prymnesum parvum (CM), Prymnesin production and the expression of the biosynthesis pathway associated polyketide synthases have been studied and metabolic trade-offs have been elucidated with transcriptomics.
Mixotrophy in the kleptoplastidic and karyokleptic Strombidium cf basimorphum (GNCM) has been studied and detailed by a combinational approache of single-cell transcriptomics, qPCR analysis and fluorescence in-situ hybridisation (FISH).
The combined approach of qPCR and FISH appears to be a promising tool to investigate the potential retention of prey nuclei in NCM.
Culture based studies allow to take samples of organisms with a known feeding history making easier the link of physiology and genomic.
Metabolic interactions of symbiont and Acantharia (eSNCM, host) of endosymbiotic mixoplankton can be explored using single-cell transcriptomics, and linked to single-cell physiological approaches (such as NanoSIMS).
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D3.7 Anestis et al (2022) Mixoplankton Genomic Toolkit v2.pdf
md5:c42f2cefa053933c7b771c1d42b690a4 |
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- Publication date:
- January 27, 2022
- DOI:
-
- Keyword(s):
- mixoplankton omics toolkit
- Grants:
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European Commission:
- MixITiN - Bringing the paradigm for marine pelagic production into the 21st century: incorporating mixotrophy into mainstream marine research (766327)
- License (for files):
- Creative Commons Attribution 4.0 International