Accurate identification of circRNA landscape and complexity reveals their pivotal roles in human oligodendroglia differentiation

CircRNA identification by A-tailing RNase R and Pseudo-reference alignment (CARP)

Circular RNAs (circRNAs) are a large class of single-stranded, stable, functional RNAs in mammalian cells having a closed-loop structure. CircRNAs occur through the covalent joining of a downstream 5′ splice donor to an upstream 3′ splice acceptor via a previously underappreciated pre-mRNA splicing mechanism, known as "back-splicing".

CARP is an intergrated 21-module computational framework designed for circRNA analysis, including circRNA identification and quantification, DE analysis, circRNA full length construction and circRNA-miRNA-mRNA network analysis based on A-tailing RNase R treated/untreated RNA-seq data.