5902965
doi
10.5281/zenodo.5902965
oai:zenodo.org:5902965
user-lory
user-lory_hslu_t_und_a
user-lory_hslu
Cerretti, Geraldine
Hochschule Luzern
Ricciardi, Dario A.
Schiffmann, David
Hochschule Luzern
Maranda, Simon
Hochschule Luzern
Kummer, Raphael
Hochschule Luzern
Zumbühl, Christoph
Hochschule Luzern
Rattenbacher-Kiser, Karin F.
Hochschule Luzern
von Arx, Silvan
Hochschule Luzern
Ammann, Sebastian
Hochschule Luzern
Strobl, Frederic
Berkane, Rayene
Hochschule Luzern
Stolz, Alexandra
Stelzer, Ernst H. K.
Egli, Marcel
Hochschule Luzern
Schleiff, Enrico
Wuest, Simon L.
Böhmer, Maik
Retrograde Analysis of Calcium Signaling by CaMPARI2 Shows Cytosolic Calcium in Chondrocytes Is Unaffected by Parabolic Flights
Hammer, Andreas
url:https://www.mdpi.com/2227-9059/10/1/138
doi:10.3390/biomedicines10010138
issn:2227-9059
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
CaMPARI; articular chondrocytes; cytosolic free calcium; gravity; parabolic flight; high throughput screening
<p>Calcium (Ca2+) elevation is an essential secondary messenger in many cellular processes, including disease progression and adaptation to external stimuli, e.g., gravitational load. Therefore, mapping and quantifying Ca2+ signaling with a high spatiotemporal resolution is a key challenge. However, particularly on microgravity platforms, experiment time is limited, allowing only a small number of replicates. Furthermore, experiment hardware is exposed to changes in gravity levels, causing experimental artifacts unless appropriately controlled. We introduce a new experimental setup based on the fluorescent Ca2+ reporter CaMPARI2, onboard LED arrays, and subsequent microscopic analysis on the ground. This setup allows for higher throughput and accuracy due to its retrograde nature. The excellent performance of CaMPARI2 was demonstrated with human chondrocytes during the 75th ESA parabolic flight campaign. CaMPARI2 revealed a strong Ca2+ response triggered by histamine but was not affected by the alternating gravitational load of a parabolic flight.</p>
+ ID der Publikation: hslu_88043 + Art des Beitrages: Wissenschaftliche Medien + Jahrgang: 2022 + Sprache: Englisch + Letzte Aktualisierung: 2022-01-25 16:01:32
Zenodo
2022-01-08
info:eu-repo/semantics/article
5902964
user-lory
user-lory_hslu_t_und_a
user-lory_hslu
1679905520.524139
4504937
md5:b0d1c756ae5062fbeeaa6c68640107c9
https://zenodo.org/records/5902965/files/Hammer_2020.pdf
public
https://www.mdpi.com/2227-9059/10/1/138
Is identical to
url
10.3390/biomedicines10010138
Is identical to
doi
2227-9059
Is published in
issn
10.5281/zenodo.5902964
isVersionOf
doi
Biomedicines
10
1
1 - 21
2022-01-08