Warming decreases host survival with multiple parasitoids, but parasitoid performance also decreases

README

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## DATASETS ##
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### data_FR.csv ### 
Daily recording of emerges from Experiment 1

- Vial.ID: unique name for each experimental vial    
- Heggs: aimed initial host density per vial (5, 10,15, 25, 50 or 100)
- Block: from 1 to 8 (same as replicate)
- T: temperature treatment (23°C or 27°C)
- Hsp: host species (BIR = D. birchii, PAL = D. pallidifrons, SIM = D. simulans)
- Psp: parasitoid species (C = controls, A = Asobara sp., L = Leptopilina sp., G = Ganaspis sp.)    
- Rep: replicate (from 1 to 8)
- H_P: H = host and P = parasitoid
- sp: species name (abbreviations: Asb = Asobara sp., Lept = Leptopilina sp., Gana = Ganaspis sp., BIR = D. birchii, PAL = D. pallidifrons, SIM = D. simulans)    
- sex: M = male and F = female (for parasitoids only, NA for hosts)
- column 6 to 46: number of emerges from day 6 to day 46 from the start of the experiment (when eggs were placed in vials) -> emerges where checked daily

### data_C.BM.csv ### 
Measurements of host body mass in controls from Experiment 1

- Vial.ID: unique name for each experimental vial    
- Heggs: initial host density (here 50 only)
- Block: from 1 to 8 (same as replicate)
- T: temperature treatment (23°C or 27°C)
- Hsp: host species (BIR = D. birchii, PAL = D. pallidifrons, SIM = D. simulans)
- Psp: parasitoid species (C = controls: no parasitoids)
- Rep: replicate (from 1 to 8)
- H_P: H = host
- column 1 to 10: individual host body mass (in µg): 10 host were weighted per vial when possible
- BM: mean host body mass from this vial (in µg)
- BM_N: number of hosts weighted from this vial

### data_FR.P.BM.csv ### 
Measurements of parasitoid body mass from Experiment 1

- Vial.ID: unique name for each experimental vial    
- Heggs: initial host density (here 50 only)
- Block: from 1 to 8 (same as replicate)
- T: temperature treatment (23°C or 27°C)
- Hsp: host species (BIR = D. birchii, PAL = D. pallidifrons, SIM = D. simulans)
- Psp: parasitoid species (Asb = Asobara sp., Lept = Leptopilina sp., Gana = Ganaspis sp.)
- Rep: replicate (from 1 to 8)
- H_P: P = parasitoid
- column 1 to 10: individual parasitoid body mass (in µg): 10 parasitoids were weighted per vial when possible
- BM: mean parasitoid body mass from this vial (in µg)
- BM_N: number of parasitoids weighted from this vial

### data_MPEexp2.csv ### 
Daily recording of emerges from Experiment 2

- Vial.ID: unique name for each experimental vial    
- Heggs: initial host density (here 50 only)
- Block: from 1 to 2
- T: temperature treatment (23°C or 27°C)
- Hsp: host species (here only D. simulans)
- Treat: H1P1 = one host and one parasitoid species, H1P2 = one host and 2 parasitoid species (always two parasitoid individuals)
- Pcomb: parasitoid assemblage (abbreviations: A = Asobara sp., L = Leptopilina sp., G = Ganaspis sp.)    
- Rep: replicate (from 1 to 8)
- H_P: H = host and P = parasitoid
- sp: species name (abbreviations: Asb = Asobara sp., Lept = Leptopilina sp., Gana = Ganaspis sp., SIM = D. simulans)    
- sex: M = male and F = female
- column 7 to 40: number of emerges from day 7 to day 40 from the start of the experiment (when eggs were placed in vials) -> emerges where checked daily

### data_MPEexp3.csv ### 
Data from dissections from Experiment 3

- T: temperature treatment (23°C or 27°C)
- Pcomb: parasitoid assemblage (abbreviations: A: Asobara sp., and G: Ganaspis sp.)    
- Block: from 1 to 2 (different than Experiment 1 and 2)
- Rep: replicate (from 1 to 12)
- Observer: name of the observer
- Dissection: time of dissection (early or late)
- Day_infection: number of days between initialization of the vial and the dissection
- H_number: larvae ID from the vial (from 1 to 10 for each vial)
- Larvae_pupae: if the host dissected was at the larval or pupal stage (we aimed to dissected larvae but it was not always possible)
- ASB_egg: number of Asobara sp. eggs
- ASB_larvae: number of Asobara sp. larvae
- ASB_melanized: number of Asobara sp. melanized eggs
- GANA_egg: number of Ganaspis sp. eggs
- GANA_larvae: number of Ganaspis sp. larvae
- GANA_melanized:  number of Ganaspis sp. melanized eggs
- NA_melanized:  number of melanized eggs not identified to species level
- Melanization_trace: if presence (y) or not (na) of other trace of melanization
- Melanization_eggs: presence (y) or not (n) of melanized eggs
- P_larvae: total number of parasitoid larvae
- P_eggs: total number of parasitoid eggs
- TOT_ASB: total number of Asobara sp.
- TOT_GANA: total number of Ganaspis sp.
- TOT_P: total number of parasitoids
- TOT_Melanized: total number of melanized eggs 
- Melanization: presence (y) or not (n) of melanization (either melanized eggs or trace of melanization)
- Super_ASB: presence (y) or not (n) of multiple Asobara sp.
- Super_GANA: presence (y) or not (n) of multiple Ganaspis sp.
- Superparasitism: presence (y) or not (n) of superparasitism
- Multiparasitism: presence (y) or not (n) of multiparasitism
- Sup_Mult: presence (y) or not (n) of either super- or multiparasitism
- ASB: presence (y) or not (n) of Asobara sp.
- GANA: presence (y) or not (n) of Ganaspis sp.
- Parasitized: presence (y) or not (n) of parasitism

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## R scripts ##
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### MPE0_data-cleaning.R ### 
R script to import, clean and modify the datasets
--> save the data needed for analyses in "MPE_data.RData"

### MPE1_FRfit.R ###
R script to fit parasitoid functional responses
--> save the results in "data.FRfitSIM.csv"
--> save the data needed for analyses in "MPE_data_FR.RData"

### MPE2_analyses1.R ### 
R script to analyze data from Experiment 2 
using the method proposed in McCoy et al. (2012). Emergent effects of multiple predators on prey survival: the importance of depletion and the functional response. Ecology letters, 15(12), 1449-1456.

### MPE3_analyses2_exp2.R ### 
R script to analyze data from Experiment 2

### MPE4_analyses3_exp3.R ### 
R script to analyze data from Experiment 3