Published January 12, 2022 | Version v0.1
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Human kidney cortex spectral confocal reference dataset 1

  • 1. University of Nebraska Medical Center
  • 2. Indiana University of School of Medicine

Description

Confocal image volume of human kidney cortex stained with antibodies for Uromodulin, AQP1, MPO, CD68, CD3 and Siglec-8 and DAPI and phalloidin.  Tissue section was mounted in Prolong Gold and imaged at 20X NA 0.75 on an Leica SP8 with spectral imaging and unmixed using LASX software.  Protocol at: 10.17504/protocols.io.9avh2.

Channels, in order:  DAPI, Phalloidin (F-Actin), Uromodulin, AQP1, MPO, CD68, CD3 and Siglec-8

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Kidney_Cortex_Human_Spectral_1.tif

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Additional details

Related works

Cites
10.17504/protocols.io.9avh2 (DOI)
Is described by
Preprint: 10.1101/2021 (DOI)

Funding

Center for Advanced Renal Microscopic Analysis 3P50DK061594-04S1
National Institutes of Health