Mashinchian, Omid
Hong, Xiaotong
Michaud, Joris
Migliavacca, Eugenia
Lefebvre, Gregory
Boss, Christophe
De Franceschi, Filippo
Le Moal, Emmeran
Collerette-Tremblay, Jasmin
Isern, Joan
Metairon, Sylviane
Raymond, Frederic
Descombes, Patrick
Bouche, Nicolas
Muñoz-Cánoves, Pura
Feige, Jerome N.
Bentzinger, C. Florian
2022-01-02
<p><strong>Abstract</strong></p>
<p>Sustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specific cell populations. Here we describe a method for the encapsulation of human and mouse skeletal muscle progenitors in diffusible polyethersulfone hollow fiber capsules that can be used to profile systemic aging in vivo independent of heterogeneous short-range tissue interactions. We observed that circulating long-range signaling factors in the old systemic environment lead to an activation of Myc and E2F transcription factors, induce senescence and suppress myogenic differentiation. Importantly, in vitro profiling using young and old serum in 2D culture does not capture all pathways deregulated in encapsulated cells in aged mice. Thus, in vivo transcriptomic profiling using cell encapsulation allows for the characterization of effector pathways of systemic aging with unparalleled accuracy.</p>
<p> </p>
https://doi.org/10.1101/2020.03.09.979054
oai:zenodo.org:5836936
Zenodo
https://zenodo.org/communities/upgrade-h2020-project
https://zenodo.org/communities/eu
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
BioRxiv, (2022-01-02)
In Vivo Transcriptomic Profiling using Cell Encapsulation Identifies Effector Pathways of Systemic Aging
info:eu-repo/semantics/preprint