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In Vivo Transcriptomic Profiling using Cell Encapsulation Identifies Effector Pathways of Systemic Aging

Mashinchian, Omid; Hong, Xiaotong; Michaud, Joris; Migliavacca, Eugenia; Lefebvre, Gregory; Boss, Christophe; De Franceschi, Filippo; Le Moal, Emmeran; Collerette-Tremblay, Jasmin; Isern, Joan; Metairon, Sylviane; Raymond, Frederic; Descombes, Patrick; Bouche, Nicolas; Muñoz-Cánoves, Pura; Feige, Jerome N.; Bentzinger, C. Florian


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{
  "description": "<p><strong>Abstract</strong></p>\n\n<p>Sustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specific cell populations. Here we describe a method for the encapsulation of human and mouse skeletal muscle progenitors in diffusible polyethersulfone hollow fiber capsules that can be used to profile systemic aging&nbsp;in vivo&nbsp;independent of heterogeneous short-range tissue interactions. We observed that circulating long-range signaling factors in the old systemic environment lead to an activation of Myc and E2F transcription factors, induce senescence and suppress myogenic differentiation. Importantly,&nbsp;in vitro&nbsp;profiling using young and old serum in 2D culture does not capture all pathways deregulated in encapsulated cells in aged mice. Thus,&nbsp;in vivo&nbsp;transcriptomic profiling using cell encapsulation allows for the characterization of effector pathways of systemic aging with unparalleled accuracy.</p>\n\n<p>&nbsp;</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland - School of Life Sciences, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), Lausanne, Switzerland", 
      "@type": "Person", 
      "name": "Mashinchian,\u00a0Omid"
    }, 
    {
      "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland - Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain", 
      "@type": "Person", 
      "name": "Hong,\u00a0Xiaotong"
    }, 
    {
      "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland - Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain", 
      "@type": "Person", 
      "name": "Michaud,\u00a0Joris"
    }, 
    {
      "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland", 
      "@type": "Person", 
      "name": "Migliavacca,\u00a0Eugenia"
    }, 
    {
      "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland", 
      "@type": "Person", 
      "name": "Lefebvre,\u00a0Gregory"
    }, 
    {
      "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland", 
      "@type": "Person", 
      "name": "Boss,\u00a0Christophe"
    }, 
    {
      "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland", 
      "@type": "Person", 
      "name": "De Franceschi,\u00a0Filippo"
    }, 
    {
      "affiliation": "D\u00e9partement de Pharmacologie-Physiologie, Institut de Pharmacologie de Sherbrooke, Centre de Recherche du Centre Hospitalier Universitaire de Sherbrooke, Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, Canada", 
      "@type": "Person", 
      "name": "Le Moal,\u00a0Emmeran"
    }, 
    {
      "affiliation": "D\u00e9partement de Pharmacologie-Physiologie, Institut de Pharmacologie de Sherbrooke, Centre de Recherche du Centre Hospitalier Universitaire de Sherbrooke, Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, Canada", 
      "@type": "Person", 
      "name": "Collerette-Tremblay,\u00a0Jasmin"
    }, 
    {
      "affiliation": "Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain", 
      "@type": "Person", 
      "name": "Isern,\u00a0Joan"
    }, 
    {
      "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland", 
      "@type": "Person", 
      "name": "Metairon,\u00a0Sylviane"
    }, 
    {
      "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland", 
      "@type": "Person", 
      "name": "Raymond,\u00a0Frederic"
    }, 
    {
      "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland", 
      "@type": "Person", 
      "name": "Descombes,\u00a0Patrick"
    }, 
    {
      "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland", 
      "@type": "Person", 
      "name": "Bouche,\u00a0Nicolas"
    }, 
    {
      "affiliation": "Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain - Department of Experimental and Health Sciences, Pompeu Fabra University (UPF), CIBER on Neurodegenerative Diseases (CIBERNED) and Instituci\u00f3 Catalana de Recerca i Estudis Avan\u00e7ats (ICREA), Barcelona, Spain", 
      "@type": "Person", 
      "name": "Mu\u00f1oz-C\u00e1noves,\u00a0Pura"
    }, 
    {
      "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland - School of Life Sciences, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), Lausanne, Switzerland", 
      "@type": "Person", 
      "name": "Feige,\u00a0Jerome N."
    }, 
    {
      "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland - D\u00e9partement de Pharmacologie-Physiologie, Institut de Pharmacologie de Sherbrooke, Centre de Recherche du Centre Hospitalier Universitaire de Sherbrooke, Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, Canada", 
      "@type": "Person", 
      "name": "Bentzinger,\u00a0C. Florian"
    }
  ], 
  "headline": "In Vivo Transcriptomic Profiling using Cell Encapsulation Identifies Effector Pathways of Systemic Aging", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2022-01-02", 
  "url": "https://zenodo.org/record/5836936", 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.1101/2020.03.09.979054", 
  "@id": "https://doi.org/10.1101/2020.03.09.979054", 
  "@type": "ScholarlyArticle", 
  "name": "In Vivo Transcriptomic Profiling using Cell Encapsulation Identifies Effector Pathways of Systemic Aging"
}
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