Preprint Open Access

In Vivo Transcriptomic Profiling using Cell Encapsulation Identifies Effector Pathways of Systemic Aging

Mashinchian, Omid; Hong, Xiaotong; Michaud, Joris; Migliavacca, Eugenia; Lefebvre, Gregory; Boss, Christophe; De Franceschi, Filippo; Le Moal, Emmeran; Collerette-Tremblay, Jasmin; Isern, Joan; Metairon, Sylviane; Raymond, Frederic; Descombes, Patrick; Bouche, Nicolas; Muñoz-Cánoves, Pura; Feige, Jerome N.; Bentzinger, C. Florian


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    "description": "<p><strong>Abstract</strong></p>\n\n<p>Sustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specific cell populations. Here we describe a method for the encapsulation of human and mouse skeletal muscle progenitors in diffusible polyethersulfone hollow fiber capsules that can be used to profile systemic aging&nbsp;in vivo&nbsp;independent of heterogeneous short-range tissue interactions. We observed that circulating long-range signaling factors in the old systemic environment lead to an activation of Myc and E2F transcription factors, induce senescence and suppress myogenic differentiation. Importantly,&nbsp;in vitro&nbsp;profiling using young and old serum in 2D culture does not capture all pathways deregulated in encapsulated cells in aged mice. Thus,&nbsp;in vivo&nbsp;transcriptomic profiling using cell encapsulation allows for the characterization of effector pathways of systemic aging with unparalleled accuracy.</p>\n\n<p>&nbsp;</p>", 
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    "title": "In Vivo Transcriptomic Profiling using Cell Encapsulation Identifies Effector Pathways of Systemic Aging", 
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    "publication_date": "2022-01-02", 
    "creators": [
      {
        "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland - School of Life Sciences, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), Lausanne, Switzerland", 
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        "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland - Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain", 
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        "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland - Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain", 
        "name": "Michaud,\u00a0Joris"
      }, 
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        "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland", 
        "name": "Migliavacca,\u00a0Eugenia"
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        "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland", 
        "name": "Lefebvre,\u00a0Gregory"
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        "name": "De Franceschi,\u00a0Filippo"
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      {
        "affiliation": "D\u00e9partement de Pharmacologie-Physiologie, Institut de Pharmacologie de Sherbrooke, Centre de Recherche du Centre Hospitalier Universitaire de Sherbrooke, Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, Canada", 
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        "affiliation": "D\u00e9partement de Pharmacologie-Physiologie, Institut de Pharmacologie de Sherbrooke, Centre de Recherche du Centre Hospitalier Universitaire de Sherbrooke, Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, Canada", 
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        "affiliation": "Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain", 
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        "affiliation": "Nestl\u00e9 Institute of Health Science, Nestl\u00e9 Research, Soci\u00e9t\u00e9 des Produits Nestl\u00e9 S.A., Lausanne, Switzerland", 
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        "affiliation": "Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain - Department of Experimental and Health Sciences, Pompeu Fabra University (UPF), CIBER on Neurodegenerative Diseases (CIBERNED) and Instituci\u00f3 Catalana de Recerca i Estudis Avan\u00e7ats (ICREA), Barcelona, Spain", 
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