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In Vivo Transcriptomic Profiling using Cell Encapsulation Identifies Effector Pathways of Systemic Aging

Mashinchian, Omid; Hong, Xiaotong; Michaud, Joris; Migliavacca, Eugenia; Lefebvre, Gregory; Boss, Christophe; De Franceschi, Filippo; Le Moal, Emmeran; Collerette-Tremblay, Jasmin; Isern, Joan; Metairon, Sylviane; Raymond, Frederic; Descombes, Patrick; Bouche, Nicolas; Muñoz-Cánoves, Pura; Feige, Jerome N.; Bentzinger, C. Florian


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{
  "DOI": "10.1101/2020.03.09.979054", 
  "container_title": "BioRxiv", 
  "author": [
    {
      "family": "Mashinchian,\u00a0Omid"
    }, 
    {
      "family": "Hong,\u00a0Xiaotong"
    }, 
    {
      "family": "Michaud,\u00a0Joris"
    }, 
    {
      "family": "Migliavacca,\u00a0Eugenia"
    }, 
    {
      "family": "Lefebvre,\u00a0Gregory"
    }, 
    {
      "family": "Boss,\u00a0Christophe"
    }, 
    {
      "family": "De Franceschi,\u00a0Filippo"
    }, 
    {
      "family": "Le Moal,\u00a0Emmeran"
    }, 
    {
      "family": "Collerette-Tremblay,\u00a0Jasmin"
    }, 
    {
      "family": "Isern,\u00a0Joan"
    }, 
    {
      "family": "Metairon,\u00a0Sylviane"
    }, 
    {
      "family": "Raymond,\u00a0Frederic"
    }, 
    {
      "family": "Descombes,\u00a0Patrick"
    }, 
    {
      "family": "Bouche,\u00a0Nicolas"
    }, 
    {
      "family": "Mu\u00f1oz-C\u00e1noves,\u00a0Pura"
    }, 
    {
      "family": "Feige,\u00a0Jerome N."
    }, 
    {
      "family": "Bentzinger,\u00a0C. Florian"
    }
  ], 
  "issued": {
    "date-parts": [
      [
        2022, 
        1, 
        2
      ]
    ]
  }, 
  "abstract": "<p><strong>Abstract</strong></p>\n\n<p>Sustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specific cell populations. Here we describe a method for the encapsulation of human and mouse skeletal muscle progenitors in diffusible polyethersulfone hollow fiber capsules that can be used to profile systemic aging&nbsp;in vivo&nbsp;independent of heterogeneous short-range tissue interactions. We observed that circulating long-range signaling factors in the old systemic environment lead to an activation of Myc and E2F transcription factors, induce senescence and suppress myogenic differentiation. Importantly,&nbsp;in vitro&nbsp;profiling using young and old serum in 2D culture does not capture all pathways deregulated in encapsulated cells in aged mice. Thus,&nbsp;in vivo&nbsp;transcriptomic profiling using cell encapsulation allows for the characterization of effector pathways of systemic aging with unparalleled accuracy.</p>\n\n<p>&nbsp;</p>", 
  "title": "In Vivo Transcriptomic Profiling using Cell Encapsulation Identifies Effector Pathways of Systemic Aging", 
  "type": "article", 
  "id": "5836936"
}
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