August 26, 2021 Journal article Open Access

Sreenivasan, Krishnamoorthy; Rodríguez-delaRosa, Alejandra; Johnny, Kim; Mesquita, Diana; Segalés, Jessica; Gomez-del Arco, Pablo; Espejo, Isabel; Ianni, Alessandro; Di Croce, Luciano; Relaix, Frederic; Redondo, Juan Miguel; Braun, Thomas; Serrano, Antonio L; Perdiguero, Eusebio; Muñoz-Cánoves, Pura

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{
  "description": "<p><strong>Summary</strong></p>\n\n<p>Regeneration of skeletal muscle requires resident stem cells called satellite cells. Here, we report that the chromatin remodeler&nbsp;<a href=\"https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/chd4\">CHD4</a>, a member of the nucleosome remodeling and deacetylase (NuRD) repressive complex, is essential for the expansion and regenerative functions of satellite cells. We show that conditional deletion of the&nbsp;<em>Chd4</em>&nbsp;gene in satellite cells results in failure to regenerate muscle after injury. This defect is principally associated with increased stem cell plasticity and lineage infidelity during the expansion of satellite cells, caused by de-repression of non-muscle-cell lineage genes in the absence of&nbsp;<em>Chd4</em>. Thus, CHD4 ensures that a transcriptional program that safeguards satellite cell identity during&nbsp;<a href=\"https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/muscle-regeneration\">muscle regeneration</a>&nbsp;is maintained. Given the therapeutic potential of muscle stem cells in diverse neuromuscular pathologies, CHD4 constitutes an attractive target for satellite cell-based therapies.</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany", 
      "@type": "Person", 
      "name": "Sreenivasan,\u00a0Krishnamoorthy"
    }, 
    {
      "affiliation": "Department of Experimental & Health Sciences, University Pompeu Fabra (UPF), CIBERNED, 08003 Barcelona, Spain", 
      "@type": "Person", 
      "name": "Rodr\u00edguez-delaRosa, Alejandra"
    }, 
    {
      "affiliation": "Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany - German Center for Cardiovascular Research (DZHK), Rhine Main, Germany", 
      "@type": "Person", 
      "name": "Johnny, Kim"
    }, 
    {
      "affiliation": "Department of Experimental & Health Sciences, University Pompeu Fabra (UPF), CIBERNED, 08003 Barcelona, Spain", 
      "@type": "Person", 
      "name": "Mesquita, Diana"
    }, 
    {
      "affiliation": "Department of Experimental & Health Sciences, University Pompeu Fabra (UPF), CIBERNED, 08003 Barcelona, Spain", 
      "@type": "Person", 
      "name": "Segal\u00e9s, Jessica"
    }, 
    {
      "affiliation": "Institute of Rare Diseases Research, Instituto de Salud Carlos III (ISCIII), 28220 Majadahonda, Madrid, Spain - Gene Regulation in Cardiovascular Remodelling & Inflammation Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 28029 Madrid, Spain", 
      "@type": "Person", 
      "name": "Gomez-del Arco, Pablo"
    }, 
    {
      "affiliation": "Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology, Barcelona, 08003 Barcelona, Spain", 
      "@type": "Person", 
      "name": "Espejo, Isabel"
    }, 
    {
      "affiliation": "Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany", 
      "@type": "Person", 
      "name": "Ianni, Alessandro"
    }, 
    {
      "affiliation": "Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology, Barcelona, 08003 Barcelona, Spain - Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany - ICREA, 08010 Barcelona, Spain", 
      "@type": "Person", 
      "name": "Di Croce, Luciano"
    }, 
    {
      "affiliation": "Univ Paris Est Creteil, INSERM, EnvA, EFS, AP-HP, IMRB, F-94010 Creteil, France", 
      "@type": "Person", 
      "name": "Relaix, Frederic"
    }, 
    {
      "affiliation": "Gene Regulation in Cardiovascular Remodelling & Inflammation Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 28029 Madrid, Spain", 
      "@type": "Person", 
      "name": "Redondo, Juan Miguel"
    }, 
    {
      "affiliation": "Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany - German Center for Cardiovascular Research (DZHK), Rhine Main, Germany - German Center for Lung Research (DZL), Giessen, Germany", 
      "@type": "Person", 
      "name": "Braun, Thomas"
    }, 
    {
      "affiliation": "Department of Experimental & Health Sciences, University Pompeu Fabra (UPF), CIBERNED, 08003 Barcelona, Spain", 
      "@type": "Person", 
      "name": "Serrano, Antonio L"
    }, 
    {
      "affiliation": "Department of Experimental & Health Sciences, University Pompeu Fabra (UPF), CIBERNED, 08003 Barcelona, Spain", 
      "@type": "Person", 
      "name": "Perdiguero, Eusebio"
    }, 
    {
      "affiliation": "Department of Experimental & Health Sciences, University Pompeu Fabra (UPF), CIBERNED, 08003 Barcelona, Spain - ICREA, 08010 Barcelona, Spain - Centro Nacional de Investigaciones Cardiovasculares (CNIC), 28029 Madrid, Spain", 
      "@type": "Person", 
      "name": "Mu\u00f1oz-C\u00e1noves, Pura"
    }
  ], 
  "headline": "CHD4 ensures stem cell lineage fidelity during skeletal muscle regeneration", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2021-08-26", 
  "url": "https://zenodo.org/record/5752328", 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.1016/j.stemcr.2021.07.022", 
  "@id": "https://doi.org/10.1016/j.stemcr.2021.07.022", 
  "@type": "ScholarlyArticle", 
  "name": "CHD4 ensures stem cell lineage fidelity during skeletal muscle regeneration"
}