5727026
doi
10.1126/sciadv.abg4119
oai:zenodo.org:5727026
Bhattacharya, Shayon
The Bernal Institute and Department of Physics, School of Natural Sciences, University of Limerick (UL), Limerick V94 T9PX, Ireland
Cazade, Pierre-André
The Bernal Institute and Department of Physics, School of Natural Sciences, University of Limerick (UL), Limerick V94 T9PX, Ireland
Molina-Osorio, Andrés F.
The Bernal Institute and Department of Chemical Sciences, School of Natural Sciences, University of Limerick (UL), Limerick V94 T9PX, Ireland
Beecher, Cillian
The Bernal Institute and Department of Chemical Sciences, School of Natural Sciences, University of Limerick (UL), Limerick V94 T9PX, Ireland
Djeghader, Ahmed
The Bernal Institute and Department of Chemical Sciences, School of Natural Sciences, University of Limerick (UL), Limerick V94 T9PX, Ireland
Soulimane, Tewfik
The Bernal Institute and Department of Chemical Sciences, School of Natural Sciences, University of Limerick (UL), Limerick V94 T9PX, Ireland
Dossot, Manuel
Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement, Université de Lorraine, CNRS, LCPME, F-54000 Nancy, France
Thompson, Damien
The Bernal Institute and Department of Physics, School of Natural Sciences, University of Limerick (UL), Limerick V94 T9PX, Ireland
Herzog, Grégoire
Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement, Université de Lorraine, CNRS, LCPME, F-54000 Nancy, France
Scanlon, Micheál D.
The Bernal Institute and Department of Chemical Sciences, School of Natural Sciences, University of Limerick (UL), Limerick V94 T9PX, Ireland
Modulating the pro-apoptotic activity of cytochrome c at a biomimetic electrified interface
Gamero-Quijano, Alonso
The Bernal Institute and Department of Chemical Sciences, School of Natural Sciences, University of Limerick (UL), Limerick V94 T9PX, Ireland
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Cytochrome c
interface between two immiscible electrolyte solutions (ITIES)
liquid|liquid interface
bioelectrochemistry
apoptosis
electrocatalysis
bio-interface
<p>Programmed cell death <em>via</em> apoptosis is a natural defence against excessive cell division, crucial for foetal development to maintenance of homeostasis and elimination of precancerous and senescent cells. Here we demonstrate an electrified liquid bio-interface that replicates the molecular machinery of the inner mitochondrial membrane at the onset of apoptosis. By mimicking <em>in vivo</em> cytochrome <em>c</em> (Cyt <em>c</em>) interactions with cell membranes, our platform allows us to modulate the conformational plasticity of the protein by simply varying the electrochemical environment at an aqueous|organic interface. Remarkably, we observe interfacial electron transfer between an organic electron donor decamethylferrocene and O<sub>2</sub>, electrocatalysed by Cyt <em>c</em>. This interfacial reaction requires partial Cyt <em>c </em>unfolding, mimicking Cyt <em>c</em> <em>in vivo </em>peroxidase activity. As proof-of-concept, we use our electrified liquid bio-interface to identify drug molecules, such as bifonazole, that can potentially downregulate Cyt <em>c</em> and protect against uncontrolled neuronal cell death in neurodegenerative disorders.</p>
D.T. thanks Science Foundation Ireland (SFI) for computing resources at the SFI/Higher Education Authority Irish Center for High-End Computing (ICHEC). A.G.-Q. acknowledges funding received from an Irish Research Council (IRC) Government of Ireland Postdoctoral Fellowship Award (Grant Number GOIPD/2018/252). G.H. and M.D. are grateful to the French Programme Investissement d'Avenir (PIA) "Lorraine Université d'Excellence" (Reference no. ANR-15-IDEX-04-LUE). M.D.S. and G.H. are grateful to the support of the IRC and Campus France for travel support between the French and Irish groups through their joint ULYSSES programme. DT thanks Science Foundation Ireland (SFI) for support (awards Grant Numbers 15/CDA/3491 and 12/RC/2275_P2).
Zenodo
2021-11-05
info:eu-repo/semantics/article
5727025
Published
award_title=Designing Reactive Functionalised Soft Interfaces _ Self-healing soft materials for solar energy conversion, energy storage, and sustainable low cost hydrogen production; award_number=13/SIRG/2137; funder_id=0271asj38; funder_name=Science Foundation Ireland;
award_title=Solar Energy Conversion without Solid State Architectures: Pushing the Boundaries of Photoconversion Efficiencies at Self-healing Photosensitiser Functionalised Soft Interfaces; award_number=716792; award_identifiers_scheme=url; award_identifiers_identifier=https://cordis.europa.eu/projects/716792; funder_id=00k4n6c32; funder_name=European Commission;
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public
Science Advances
7
45
eabg4119
2021-11-05