Journal article Open Access

DEVELOPMENT AND VALIDATION FOR ESTIMATION OF CLINDAMYCIN, ADAPALENE AND SOFOSBUVIR IN BULK AND PHARMACEUTICAL DOSAGE FORMS BY RP-HPLC METHOD

G. Swathi*, G.Deepthi , Deepthi Visakh, J. Prathyusha, Nafees Fatima, M. Sai lakshmi


DataCite XML Export

<?xml version='1.0' encoding='utf-8'?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd">
  <identifier identifierType="DOI">10.5281/zenodo.5718586</identifier>
  <creators>
    <creator>
      <creatorName>G. Swathi*, G.Deepthi , Deepthi Visakh, J. Prathyusha, Nafees Fatima, M. Sai lakshmi</creatorName>
    </creator>
  </creators>
  <titles>
    <title>DEVELOPMENT AND VALIDATION FOR ESTIMATION OF   CLINDAMYCIN, ADAPALENE AND SOFOSBUVIR IN BULK AND PHARMACEUTICAL DOSAGE FORMS BY RP-HPLC METHOD</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-11-22</date>
  </dates>
  <resourceType resourceTypeGeneral="JournalArticle"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/5718586</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.5718585</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;&lt;em&gt;A simple, accurate, rapid and precise isocratic stability indicating reversed-phase high-performance liquid chromatographic method has been developed and validated for simultaneous determination of Clindamycin and Adapalene in tablets. The chromatographic separation was carried out on C&lt;sub&gt;18&lt;/sub&gt; BDS Hypersil (150 x 4.6mm, 5&amp;micro;)&amp;nbsp; &amp;nbsp;with a mixture ofmixed phosphate buffer : acetonitrile (55:45%v/v) &amp;nbsp;as a mobile phase at a flow rate of 1.0mL/min. UV detection was performed at 230nm. The retention times were2.84 and 3.999min for Clindamycin and Adapalene respectively. Calibration plots were linear (r&lt;sup&gt;2&lt;/sup&gt;=0.999) over the concentration range of 25-150&amp;micro;g/mL for Clindamycinand2.5-15&amp;micro;g/mL for Adapalene. The method was validated for accuracy, precision, specificity, linearity and sensitivity. The proposed method was successfully used for quantitative analysis of tablets. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for routine determination of Clindamycin and Adapalene in bulk and tablet dosage form.&lt;/em&gt;&lt;/p&gt;

&lt;p&gt;&lt;em&gt;Sofosbuvir is used primarily to treat&amp;nbsp;hepatitis C&amp;nbsp;and&amp;nbsp;viral hemorrhagic fevers. It is possible to select a sofosbuvir resistant mutant of HCV that can replicate to levels similar to wild type virus grown without sofosbuvir. Analysis of the mutations responsible for the sofosbuvir resistance may aid in understanding the mechanism of action of sofosbuvir.&lt;/em&gt;&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Keywords: &lt;/strong&gt;&lt;em&gt;Clindamycin , Adapalene, RP-HPLC, Tablets, hepatitis C.&lt;/em&gt;&lt;/p&gt;</description>
  </descriptions>
</resource>
20
9
views
downloads
All versions This version
Views 2020
Downloads 99
Data volume 8.2 MB8.2 MB
Unique views 2020
Unique downloads 99

Share

Cite as