Dataset Open Access
The LungMAP scRNA-Seq reference associated with the Lung CellCards resource. The initial reference integrated 259k cells from 72 donors from five published (PMIDs: 32726565, 32427931, 30554520, 32832599, 32832598) and one unpublished single cell RNA-seq cohort. Non-diseased adult and pediatric healthy lung single-cell 10x Genomics captures (3’ v2 and v3). Cells from different donors were integrated using Batchlor. Preliminary cell types were called based on Leiden clustering analysis and expression patterns of LungMAP cell card markers. UMAP embeddings were generated using monocle3. This reference, along with a corresponding single-nucleus specific version of this atlas, is under active construction. We expect to release the beta version of the reference in November 2021. Conforms to Azimuth reference data structure described at https://github.com/satijalab/azimuth/wiki/Azimuth-Reference-Format.