PHYTOCHEMICAL EVALUATION & PHARMACOLOGICAL SCREENING OF ANTIDIABETIC ACTIVITY USING ETHANOLIC EXTRACTS OF PULICARIA DYSENTERICA & BROSIMUM ALICASTRUM ON EXPERIMENTAL ANIMALS

This project is focused on the study of anti-diabetic effects of the leaf extract of Pulicaria dysentrica &brosimum alicastrum on streptozotocin induced male Wister rats. To evaluate the anti-diabetic activity of ethanolic extract of pulicaria dysentrica & brosimum alicastrum, this is tested by phytochemical analysis, GCMS analysis, In vivo & Invitro screening models. Oral dosing of plants extracts in different doses alleviates rats blood serum levels. This may be achieved by estimating the BGL, SGOT, SGPT, TGR, TCL, ALP, HDL, VLDL, LDL and the histopathological study of the pancreas. The rats’ pancreas was dissected out from one animal of each group for histopathology examination for evaluation of hyperplasia, islets damage, and restoration of size of organ. The pancreas microscopy of toxic, standard &toxic group is compared. It was found that with the plant extract the hyperplasia is repairing when compared to toxic but is less compared to standard control. The study asserts that further research needs to be undertaken on the benefits of pulicaria dysentrica and brosimum alicastrum in diabetes. Keywords: anti-diabetes, ethanol, streptozotocin, pulicaria dysentrica, brosimum alicastrum, hyperplasia.