Published September 30, 2021 | Version v1
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Markers of atherosclerosis lipoprotein-associated phospholipase A2 and E-selectin and vascular risk factors in patients with carotid stenosis

Creators

  • 1. Kharkiv Medical Academy of Postgraduate Education, Ukraine

Description

The aim of this study is to evaluate serum level biomarkers of atherosclerosis lipoprotein-associated phospholipase A2 and E-selectin in patients with atherosclerotic carotid stenosis with different clinical manifestation in associated with vascular risk factors.

Materials and methods: A total 106 patients with atherosclerotic carotid stenosis (74 men and 32 women, aged from 31 to 74 years, mean 62.6±0.9) were included: with acute ipsilateral atherothrombotic stroke (35), history of stroke and carotid endarterectomy (41) and 30 patients with asymptomatic carotid stenosis. The control group consist of 20 health subjects without cardiovascular disease. All participants underwent duplex sonography. Lipoprotein-associated phospholipase A2 and E-selectin was measured using commercially available (ELISA) kit.

Results: The level of lipoprotein-associated phospholipase A2 was in general 55.664±3.537 ng/ml, which was significantly higher (M-W U=10, p=1.023136´10-11 <0.05) than in the control group (9.296±0.935 ng/ml). Level was significantly higher in groups of symptomatic patients who underwent carotid endarterectomy (p=0.04893), and proportion patients with high degree stenosis >70 % was greater in this group. The level of E-selectin in the study patients was significantly higher (7.653±0.246 pg/ml) than in the control group (3.101±0.503 pg/ml) p<0.05. No association the serum level of lipoprotein-associated phospholipase A2 and E-selectin with common stroke risk factor such as hypercholesterinemia, smoking and body mass index were found, but positive correlation of lipoprotein-associated phospholipase A2 with E-selectin was significant (p=0.00085).

Conclusions: Increasing plasma level lipoprotein-associated phospholipase A2 and E-selectin in patients with the carotid atherosclerotic stenosis were observe. Statistically significant correlation between the level of lipoprotein-associated phospholipase A2 and E-selectin were found in symptomatic carotid atherosclerotic stenosis

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References

  • Den Hartog, A. G., Achterberg, S., Moll, F. L., Kappelle, L. J., Visseren, F. L. J., van der Graaf, Y. et. al. (2013). Asymptomatic Carotid Artery Stenosis and the Risk of Ischemic Stroke According to Subtype in Patients With Clinical Manifest Arterial Disease. Stroke, 44 (4), 1002–1007. doi: http://doi.org/10.1161/strokeaha.111.669267
  • Puig, N., Jiménez-Xarrié, E., Camps-Renom, P., Benitez, S. (2020). Search for Reliable Circulating Biomarkers to Predict Carotid Plaque Vulnerability. International Journal of Molecular Sciences, 21 (21), 8236. doi: http://doi.org/10.3390/ijms21218236
  • Martinez, E., Martorell, J., Riambau, V. (2020). Review of serum biomarkers in carotid atherosclerosis. Journal of Vascular Surgery, 71 (1), 329–341. doi: http://doi.org/10.1016/j.jvs.2019.04.488
  • Mayer, F. J., Binder, C. J., Wagner, O. F., Schillinger, M., Minar, E., Mlekusch, W. et. al. (2016). Combined Effects of Inflammatory Status and Carotid Atherosclerosis. Stroke, 47 (12), 2952–2958. doi: http://doi.org/10.1161/strokeaha.116.013647
  • Liu, J., Wang, W., Qi, Y., Yong, Q., Zhou, G., Wang, M. et. al. (2014). Association between the Lipoprotein-Associated Phospholipase A2 Activity and the Progression of Subclinical Atherosclerosis. Journal of Atherosclerosis and Thrombosis, 21 (6), 532–542. doi: http://doi.org/10.5551/jat.20941
  • Toth, P. P., McCullough, P. A., Wegner, M. S., Colley, K. J. (2010). Lipoprotein-associated phospholipase A2: role in atherosclerosis and utility as a cardiovascular biomarker. Expert Review of Cardiovascular Therapy, 8 (3), 425–438. doi: http://doi.org/10.1586/erc.10.18
  • Ding, G., Wang, J., Liu, K., Huang, B., Deng, W., He, T. (2020). Association of E-Selectin gene rs5361 polymorphism with ischemic stroke susceptibility: a systematic review and Meta-analysis. International Journal of Neuroscience, 131 (5), 511–517. doi: http://doi.org/10.1080/00207454.2020.1750385
  • Ammirati, E., Moroni, F., Norata, G. D., Magnoni, M., Camici, P. G. (2015). Markers of Inflammation Associated with Plaque Progression and Instability in Patients with Carotid Atherosclerosis. Mediators of Inflammation, 2015, 1–15. doi: http://doi.org/10.1155/2015/718329
  • Zemlin, A. E., Matsha, T. E., Kengne, A. P., Hon, G. M., Erasmus, R. T. (2016). Correlation of E-selectin concentrations with carotid intima-media thickness and cardio-metabolic profile of mixed ancestry South Africans: a cross-sectional study. Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 54 (1), 92–100. doi: http://doi.org/10.1177/0004563216640001
  • Rothwell, P. M., Gutnikov, S. A., Warlow, C. P. (2003). Reanalysis of the Final Results of the European Carotid Surgery Trial. Stroke, 34 (2), 514–523. doi: http://doi.org/10.1161/01.str.0000054671.71777.c7
  • Wijeratne, T., Menon, R., Sales, C., Karimi, L., Crewther, S. (2020). Carotid artery stenosis and inflammatory biomarkers: the role of inflammation-induced immunological responses affecting the vascular systems. Annals of Translational Medicine, 8 (19), 1276. doi: http://doi.org/10.21037/atm-20-4388
  • Ma, Y. (2015). Associations of platelet-activating factor acetylhydrolase gene polymorphisms with risk of ischemic stroke. Biomedical Reports, 4 (2), 246–250. doi: http://doi.org/10.3892/br.2015.560
  • Younus, A., Humayun, C., Ahmad, R., Ogunmoroti, O., Kandimalla, Y., Aziz, M. et. al. (2017). Lipoprotein-associated phospholipase A2 and its relationship with markers of subclinical cardiovascular disease: A systematic review. Journal of Clinical Lipidology, 11 (2), 328–337. doi: http://doi.org/10.1016/j.jacl.2017.02.005
  • Hu, G., Liu, D., Tong, H., Huang, W., Hu, Y., Huang, Y. (2019). Lipoprotein-Associated Phospholipase A2 Activity and Mass as Independent Risk Factor of Stroke: A Meta-Analysis. BioMed Research International, 2019, 1–11. doi: http://doi.org/10.1155/2019/8642784
  • Sarlon-Bartoli, G., Boudes, A., Buffat, C., Bartoli, M. A., Piercecchi-Marti, M. D., Sarlon, E. et. al. (2012). Circulating Lipoprotein-associated Phospholipase A2 in High-grade Carotid Stenosis: A New Biomarker for Predicting Unstable Plaque. Journal of Vascular Surgery, 55 (2), 617. doi: http://doi.org/10.1016/j.jvs.2011.12.032
  • Yang, Y., Xue, T., Zhu, J., Xu, J., Hu, X., Wang, P. et. al. (2017). Serum lipoprotein-associated phospholipase A2 predicts the formation of carotid artery plaque and its vulnerability in anterior circulation cerebral infarction. Clinical Neurology and Neurosurgery, 160, 40–45. doi: http://doi.org/10.1016/j.clineuro.2017.06.007
  • Wang, C., Fang, X., Hua, Y., Liu, Y., Zhang, Z., Gu, X. et. al. (2017). Lipoprotein-Associated Phospholipase A2 and Risk of Carotid Atherosclerosis and Cardiovascular Events in Community-Based Older Adults in China. Angiology, 69 (1), 49–58. doi: http://doi.org/10.1177/0003319717704554
  • Sakurai, S., Kitamura, A., Cui, R., Yamagishi, K., Tanigawa, T., Iso, H. (2009). Relationships of Soluble E-Selectin and High-Sensitivity C-Reactive Protein with Carotid Atherosclerosis in Japanese Men. Journal of Atherosclerosis and Thrombosis, 16 (4), 339–345. doi: http://doi.org/10.5551/jat.no182