COBALT OXIDE NANOPARTICLES: SYNTHESIS AND CHARACTERIZATION

The cobalt oxide Nanoparticles were synthesized from Cobalt Nitrate Hexahydrate aqueous solution under the chemical method at 60-90°C.  The average crystallite size was calculated from De-Bye Scherrer’s equation. FESEM, EDX, XRD were used to characterize the structural features of the product. FTIR spectra confirmed the adsorption of the  cobalt oxide nanoparticles. In addition, UV-visible absorption spectra were employed to estimate the band gap energy of the copper oxide nanoparticles. This method may be suitable for large scale production of cobalt oxide nanoparticles for practical applications. The effect of cobalt oxide nanoparticles is screened in vitro for antimicrobial activity by Disc diffusion method. The bacterial organisms used in this study are E.coli, Bascillus Subtilis. The observed inhibition zones for these nanoparticles are in the range of 22mm for E.coli and 20mm for Bascillus Subtilis. The cytotoxicity activities of copper oxide nanoparticles screened by MTT-assay. We have screened for one type of cancer cell-line i.e MCF-7( Breast Cancer), cobalt oxide nanoparticles obtained IC₅₀ values in the range of 54ug/ml for MCF-7 cell line.

Keywords: Cobalt Oxide Nanoparticles, SEM, EDX, XRD,FTIR,UV-Vis, Disc diffusion method, Cytotoxicity.