Journal article Open Access

Impact of Diuretics on Metabolic Activity of Urogenital Tract Microbiota in Women

Amar Balihodžić; Nadira Ibrišimović Mehmedinović; Suada Tinjić; Semira Galijašević; Kamelija Madacki-Todorović; Izet Eminović; Lejla Hasanbegović; Mirza Ibrišimović


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{
  "inLanguage": {
    "alternateName": "eng", 
    "@type": "Language", 
    "name": "English"
  }, 
  "about": [
    {
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      "@type": "CreativeWork"
    }, 
    {
      "@id": "https://hdl.handle.net/100.1/ijeat.F29880810621", 
      "@type": "CreativeWork"
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  ], 
  "description": "<p>Limited knowledge exists about the effects of commonly used diuretic medications on the human normal flora. Thus, we investigated potential stimulatory effects of diuretic drug furosemide on urogenital tract microbiota in women. Three strains of E. coli and C. albicans with different biofilm forming capacities were obtained from female patients diagnosed with urinary tract infections. All tested strains were treated with two different concentrations of furosemide drug, in comparison to non-treated strains as the negative control. At specific time intervals, samples were obtained from growing culture and analyzed for their proliferation rate, aspartyl proteinase excretion and biofilm formation ability. E. coli and C. albicans strains significantly increased their aspartyl proteinase excretion under furosemide treatment. This effect was frequently observed after 16 hours of incubation at 37oC. This drug has also increased the biofilm forming capacities of E. coli and C. albicans strains. Interestingly, both E. coli and C. albicans non-biofilm former strains, gained the capacity of biofilm formation when treated with furosemide at certain concentrations. E. coli control became a weak biofilm former after 48 hours of incubation, while non-biofilm former C. albicans strain became a weak biofilm former in dose-dependent fashion, after 48 hours incubation with furosemide in concentration of 0.1 mg/mL, and after 16 hours of incubation with furosemide in concentration of 0.5 mg/mL. Loop diuretic drug furosemide is able to increase the microbial virulence and turn commensal microbes into opportunistic pathogens. Additionally, the results suggest that enzyme aspart</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Sarajevo Medical School, University Sarajevo School of Science and Technology, Sarajevo, Bosnia and Herzegovina", 
      "@type": "Person", 
      "name": "Amar Balihod\u017ei\u0107"
    }, 
    {
      "affiliation": "Department of Chemistry, Faculty of Natural Science and Mathematics, University of Tuzla, Tuzla, Bosnia and Herzegovina.", 
      "@type": "Person", 
      "name": "Nadira Ibri\u0161imovi\u0107 Mehmedinovi\u0107"
    }, 
    {
      "affiliation": "Gynecology Polyclinic \"Korak do \u017eivota\", Tuzla, Bosnia and Herzegovina.", 
      "@type": "Person", 
      "name": "Suada Tinji\u0107"
    }, 
    {
      "affiliation": "Sarajevo Medical School, University Sarajevo School of Science and Technology, Sarajevo, Bosnia and Herzegovina.", 
      "@type": "Person", 
      "name": "Semira Galija\u0161evi\u0107"
    }, 
    {
      "affiliation": "Sarajevo Medical School, University Sarajevo School of Science and Technology, Sarajevo, Bosnia and Herzegovina.", 
      "@type": "Person", 
      "name": "Kamelija Madacki-Todorovi\u0107"
    }, 
    {
      "affiliation": "Faculty of Natural Science and Mathematics, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.", 
      "@type": "Person", 
      "name": "Izet Eminovi\u0107"
    }, 
    {
      "affiliation": "Biochemical-Immunological-Hemathological \"Medical Laboratory\" Ilid\u017ea, Sarajevo, Bosnia and Herzegovina.", 
      "@type": "Person", 
      "name": "Lejla Hasanbegovi\u0107"
    }, 
    {
      "affiliation": "Gynecology Polyclinic \"Korak do \u017eivota\",Tuzla, Bosnia and Herzegovina and Sarajevo Medical School, University Sarajevo School of Science and Technology.", 
      "@type": "Person", 
      "name": "Mirza Ibri\u0161imovi\u0107"
    }
  ], 
  "headline": "Impact of Diuretics on Metabolic Activity of Urogenital Tract Microbiota in Women", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2021-10-30", 
  "keywords": [
    "Additionally, the results suggest that enzyme aspart"
  ], 
  "url": "https://zenodo.org/record/5502326", 
  "contributor": [
    {
      "affiliation": "Publisher", 
      "@type": "Person", 
      "name": "Blue Eyes Intelligence Engineering and Sciences Publication(BEIESP)"
    }
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.35940/ijeat.F2988.10110121", 
  "@id": "https://doi.org/10.35940/ijeat.F2988.10110121", 
  "@type": "ScholarlyArticle", 
  "name": "Impact of Diuretics on Metabolic Activity of Urogenital Tract Microbiota in Women"
}
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